Voluntary alcohol consumption alters stress-induced changes in dopamine-2 receptor binding in Wistar-Kyoto rat brain.
Voluntary alcohol consumption alters stress-induced changes in dopamine-2 receptor binding in Wistar-Kyoto rat brain.
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DOI：
10.1016/j.pbb.2009.10.010
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发表时间：
2010-01
影响因子：
3.6
通讯作者：
Tejani-Butt, Shanaz M.
中科院分区：
文献类型：
--
作者：
Yaroslavsky, Irene;Tejani-Butt, Shanaz M.
Wistar-Kyoto（WKY）大鼠已被提议作为抑郁行为的动物模型，与其他大鼠品系相比，其对应激刺激表现出高反应性。我们已经证明，WKY大鼠消耗200%以上的酒精在天真的条件下相比，其远系繁殖的同行，Wistar（WIS）大鼠。本研究旨在了解这两种行为不同的大鼠品系的压力和酒精消耗对中枢多巴胺2型（D2）受体位点的影响。本研究的第一部分检查了慢性应激对饮酒的影响，而第二部分检查了[125 I]-碘舒必利与对照、应激或应激和酒精联合治疗的WKY大鼠与WIS大鼠中D2受体的结合。暴露于慢性应激导致两种大鼠品系的酒精消耗量增加，WKY大鼠在有或没有应激暴露的情况下比WIS大鼠消耗更多的酒精。定量放射自显影实验表明，慢性应激使WKY大鼠尾壳核（CPu）、中脑核（NAc）、黑质（SN）和腹侧被盖区（VTA）的D2受体结合增加，而使WIS大鼠尾壳核和黑质的D2受体结合减少。与应激动物相比，WKY大鼠与压力和酒精共同治疗表现出减少D2受体位点的细胞体区域（SN和VTA），而WIS大鼠受体结合没有变化。观察到的D2受体位点的变化可能表明应激和酒精暴露后DA神经传递的改变。由于应激的WKY大鼠消耗更多的酒精，因此饮酒可能会逆转细胞体区域中应激诱导的D2受体变化，这表明存在自我用药表型。
The Wistar-Kyoto (WKY) rat has been proposed as an animal model of depressive behavior and exhibits hyper-responsiveness to stressful stimulation when compared to other rat strains. We have demonstrated that WKY rats consume 200% more alcohol under naïve conditions as compared to their outbred counterparts, Wistar (WIS) rats. The present study was designed to understand the influence of stress and alcohol consumption on central dopamine type-2 (D2) receptor sites in these two behaviorally distinct rat strains. The first part of this study examined the effects of chronic stress on alcohol consumption, while the second part examined the binding of [125I]-Iodosulpiride to D2 receptors in control, stressed or stress and alcohol co-treated WKY compared to WIS rats. Exposure to chronic stress led to an increase in the amount of alcohol consumed by both rat strains, with WKY rats consuming significantly more alcohol than WIS rats with or without stress exposure. Quantitative autoradiography experiments showed that chronic stress increased D2 receptor binding in the caudate putamen (CPu), nucleus accumbens (NAc), substantia nigra (SN) and ventral tegmental area (VTA) of WKY rats, and reduced receptor binding in the CPu and SN of WIS rats. Compared to the stressed-animals, WKY rats co-treated with stress and alcohol demonstrated a reduction in D2 receptor sites in the cell body regions (SN and VTA), while WIS rats showed no changes in receptor binding. The observed changes in D2 receptor sites may indicate altered DA neurotransmission following stress and alcohol exposure. Since stressed WKY rats consumed more alcohol, it is possible that consumption of alcohol reverses the stress-induced D2 receptor alterations in the cell body regions, suggestive of a self medicating phenotype.
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影响因子：
2.5
作者：
FALLON, JH;MOORE, RY
通讯作者：
MOORE, RY
影响因子：
2.9
作者：
IMPERATO, A;PUGLISIALLEGRA, S;ANGELUCCI, L
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ANGELUCCI, L
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作者：
CARLI, M;EVENDEN, JL;ROBBINS, TW
通讯作者：
ROBBINS, TW
影响因子：
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作者：
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通讯作者：
Tejani-Butt, SM
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作者：
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通讯作者：
De Montis, MG