Change of cytokeratin filament organization during the cell cycle: selective masking of an immunologic determinant in interphase PtK2 cells.
Change of cytokeratin filament organization during the cell cycle: selective masking of an immunologic determinant in interphase PtK2 cells.
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DOI：
10.1083/jcb.97.4.1255
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发表时间：
1983-10
期刊：
影响因子：
--
通讯作者：
Geiger B
中科院分区：
文献类型：
--
作者：
Franke WW;Schmid E;Wellsteed J;Grund C;Gigi O;Geiger B
在PtK2细胞的培养中，用一种单抗(KG8.13)研究了中等大小的角蛋白型细丝(IF)的组织结构。在免疫印迹实验中，该抗体与这些细胞中存在的两种主要细胞角蛋白多肽中较大的一个发生反应，但使用标准的免疫荧光显微镜程序，不与间期细胞中丰富的细胞角蛋白细丝反应，这与各种抗血清和其他单抗形成鲜明对比。然而，在相同的细胞培养中，该抗体确实与有丝分裂细胞和有丝分裂后早期细胞的角蛋白细丝发生反应。与有丝分裂细胞角蛋白细丝的特异性反应仅是由于有丝分裂中特异性免疫决定子的暴露及其在间期细胞中的遮蔽。用Triton X-100以及甲醇和丙酮处理间期细胞会改变细胞角蛋白细丝，并允许它们与这种单抗发生反应。在含有2M尿素或低浓度胰酶的缓冲液处理后，也观察到类似的去掩蔽现象。我们的结论是，在PtK2细胞的有丝分裂过程中，尽管细胞角蛋白的组织仍然排列在典型的IF中，但它的组织发生了变化。
The organization of intermediate-sized filaments (IF) of the cytokeratin type was studied in cultures of PtK2 cells in which typical IF structures are maintained during mitosis, using a monoclonal antibody (KG 8.13). This antibody reacts, in immunoblotting experiments, with the larger of the two major cytokeratin polypeptides present in these cells but, using standard immunofluorescence microscopy procedures, does not react with the cytokeratin filaments abundant in interphase cells, in striking contrast to various antisera and other monoclonal cytokeratin antibodies. In the same cell cultures, however, the antibody does react with cytokeratin filaments of mitotic and early postmitotic cells. The specific reaction with cytokeratin filaments of mitotic cells only is due to the exposure of the specific immunologic determinant in mitosis and its masking in interphase cells. Treatment of interphase cells with both Triton X-100 as well as with methanol and acetone alters the cytokeratin filaments and allows them to react with this monoclonal antibody. A similar unmasking was noted after treatment with buffer containing 2 M urea or low concentrations of trypsin. We conclude that the organization of cytokeratin, albeit still arranged in typical IF, is altered during mitosis of PtK2 cells.
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影响因子：
7.8
作者：
FRANKE, WW;SCHMID, E;KEENAN, TW
通讯作者：
KEENAN, TW
DOI：
10.1073/pnas.76.7.3372
发表时间：
1979-01-01
影响因子：
11.1
作者：
BLOSE, SH
通讯作者：
BLOSE, SH
影响因子：
5.6
作者：
BRAVO, R;SMALL, JV;CELIS, JE
通讯作者：
CELIS, JE
影响因子：
3.7
作者：
HORWITZ, B;KUPFER, H;GEIGER, B
通讯作者：
GEIGER, B
影响因子：
3.7
作者：
FRANKE, WW;SCHMID, E;WEBER, K
通讯作者：
WEBER, K