Recruiting TATA-binding protein to a promoter: transcriptional activation without an upstream activator
Recruiting TATA-binding protein to a promoter: transcriptional activation without an upstream activator
复制标题
将 TATA 结合蛋白招募到启动子：无需上游激活剂的转录激活
DOI：
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发表时间：
1995
影响因子：
5.3
通讯作者：
John T. Lis
中科院分区：
文献类型：
--
作者：
Hua Xiao;J. Friesen;John T. Lis
The binding of TATA-binding protein (TBP) to the TATA element is the first step in the initiation of RNA polymerase II transcription from many promoters in vitro. It has been proposed that upstream activator proteins stimulate transcription by recruiting TBP to the promoter, thus facilitating the assembly of a transcription complex. However, the role of activator proteins acting at this step to stimulate transcription in vivo remains largely speculative. To test whether recruitment of TBP to the promoter is sufficient for transcriptional activation in vivo, we constructed a hybrid protein containing TBP of the yeast Saccharomyces cerevisiae fused to the DNA-binding domain of GAL4. Our results show that TBP recruited by the GAL4 DNA-binding domain to promoters bearing a GAL4-binding site can interact with the TATA element and direct high levels of transcription. This finding indicates that binding of TBP to promoters in S. cerevisiae is a major rate-limiting step accelerated by upstream activator proteins.
影响因子：
10.5
作者：
Weidong Wang;J. Gralla;Michael F. Carey
通讯作者：
Weidong Wang;J. Gralla;Michael F. Carey
酿酒酵母 CYC1 基因的两种 TATA 元件。
影响因子：
5.3
作者：
Li,WZ;Sherman,F
通讯作者：
Sherman,F
通过体内转录激活域增加 TATA 结合蛋白向启动子的募集。
DOI：
10.1126/science.7939664
发表时间：
1994
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Klein,C;Struhl,K
通讯作者：
Struhl,K