Temperature regulates transcription in the zebrafish circadian clock.
Temperature regulates transcription in the zebrafish circadian clock.
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DOI：
10.1371/journal.pbio.0030351
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发表时间：
2005-11
期刊：
影响因子：
9.8
通讯作者：
Foulkes NS
中科院分区：
文献类型：
--
作者：
Lahiri K;Vallone D;Gondi SB;Santoriello C;Dickmeis T;Foulkes NS
温度影响生物钟的关键方面，这一点已经得到充分的证明。温度周期会影响生物钟，而昼夜节律周期的周期长度会被调整，使其在较宽的温度范围内保持相对恒定（温度补偿）。在脊椎动物中，这些特性的分子基础知之甚少。在这里，使用斑马鱼作为一个变温模型，我们首先证明，在没有光的情况下，暴露的胚胎和原代细胞系的温度周期夹带时钟基因表达的昼夜节律。温度的步骤驱动某些时钟基因的基础表达的变化，在基因特异性的方式，一种机制可能有助于夹带。在per 4基因的情况下，虽然E-box启动子元件介导生物钟调节，但它们不指导转录中的温度驱动变化。其次，通过研究E-box调控的转录作为核心时钟机制的报告者，我们揭示了斑马鱼的时钟是温度补偿的。此外，温度强烈影响昼夜转录节律的振幅期间和以下夹带的光暗周期，一个属性，可以赋予温度补偿。最后，我们展示了时钟蛋白CLK的表达水平、磷酸化和功能的温度依赖性变化。这表明了一种机制，可以解释E盒定向节律的振幅变化。总之，我们的研究结果表明，在斑马鱼时钟的核心几个关键的转录调控元件响应温度。揭示了温度周期诱导斑马鱼生物钟基因表达昼夜节律的分子基础
It has been well-documented that temperature influences key aspects of the circadian clock. Temperature cycles entrain the clock, while the period length of the circadian cycle is adjusted so that it remains relatively constant over a wide range of temperatures (temperature compensation). In vertebrates, the molecular basis of these properties is poorly understood. Here, using the zebrafish as an ectothermic model, we demonstrate first that in the absence of light, exposure of embryos and primary cell lines to temperature cycles entrains circadian rhythms of clock gene expression. Temperature steps drive changes in the basal expression of certain clock genes in a gene-specific manner, a mechanism potentially contributing to entrainment. In the case of the per4 gene, while E-box promoter elements mediate circadian clock regulation, they do not direct the temperature-driven changes in transcription. Second, by studying E-box-regulated transcription as a reporter of the core clock mechanism, we reveal that the zebrafish clock is temperature-compensated. In addition, temperature strongly influences the amplitude of circadian transcriptional rhythms during and following entrainment by light–dark cycles, a property that could confer temperature compensation. Finally, we show temperature-dependent changes in the expression levels, phosphorylation, and function of the clock protein, CLK. This suggests a mechanism that could account for changes in the amplitude of the E-box-directed rhythm. Together, our results imply that several key transcriptional regulatory elements at the core of the zebrafish clock respond to temperature. Reveals the molecular basis by which temperature cycles entrain circadian rhythms of clock gene expression in zebrafish
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DOI：
10.1101/sqb.1960.025.01.015
发表时间：
1960-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
PITTENDRIGH, CS
通讯作者：
PITTENDRIGH, CS
影响因子：
9.8
作者：
Kaneko M;Cahill GM
通讯作者：
Cahill GM
影响因子：
9.2
作者：
Cermakian, N;Pando, MP;Sassone-Corsi, P
通讯作者：
Sassone-Corsi, P
影响因子：
3.5
作者：
PITTENDRIGH, CS;KYNER, WT;TAKAMURA, T
通讯作者：
TAKAMURA, T
DOI：
10.1098/rstb.1990.0037
发表时间：
1990-01-30
影响因子：
6.3
作者：
JOHNSTON, IA
通讯作者：
JOHNSTON, IA