Light-dependent development of circadian gene expression in transgenic zebrafish.
Light-dependent development of circadian gene expression in transgenic zebrafish.
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DOI：
10.1371/journal.pbio.0030034
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发表时间：
2005-02
期刊：
影响因子：
9.8
通讯作者：
Cahill GM
中科院分区：
文献类型：
--
作者：
Kaneko M;Cahill GM
环境刺激在发育过程中启动和同步昼夜节律振荡的作用在不同的节律过程中似乎是不同的。在斑马鱼中，幼体只有在暴露于明暗循环(LD)后才会出现各种节律，而斑马鱼Period 3(PER3)mRNA在恒定的条件下从发育的第一天起就有节律。我们获得了转基因斑马鱼，其中萤火虫荧光素酶(Luc)基因的表达由斑马鱼PER3启动子驱动。来自这些品系的活幼虫具有节律性的生物发光，为高通量测量体内昼夜节律基因表达提供了第一个脊椎动物系统。在恒定条件下的昼夜节律性仅在发育后5-6d观察到，只有在第4天后暴露在LD信号下才能观察到。无论光照如何，都观察到了一种新的发育曲线，最初几天表达很低，当活动游泳开始时迅速增加。环境温度影响PER3和Luc mRNA的发育概况和总体水平，以及需要LD循环才能产生强劲的生物发光节律的关键天数。总之，PER3-Luc斑马鱼在时钟基因的表达中揭示了发育事件、光和温度之间的复杂相互作用。在时钟基因启动子下表达萤火虫荧光素酶基因的斑马鱼的产生使体内分子昼夜节律的研究成为可能
The roles of environmental stimuli in initiation and synchronization of circadian oscillation during development appear to vary among different rhythmic processes. In zebrafish, a variety of rhythms emerge in larvae only after exposure to light-dark (LD) cycles, whereas zebrafish period3 (per3) mRNA has been reported to be rhythmic from day 1 of development in constant conditions. We generated transgenic zebrafish in which expression of the firefly luciferase (luc) gene is driven by the zebrafish per3 promoter. Live larvae from these lines are rhythmically bioluminescent, providing the first vertebrate system for high-throughput measurement of circadian gene expression in vivo. Circadian rhythmicity in constant conditions was observed only after 5–6 d of development, and only if the fish were exposed to LD signals after day 4. Regardless of light exposure, a novel developmental profile was observed, with low expression during the first few days and a rapid increase when active swimming begins. Ambient temperature affected the developmental profile and overall levels of per3 and luc mRNA, as well as the critical days in which LD cycles were needed for robust bioluminescence rhythms. In summary, per3-luc zebrafish has revealed complex interactions among developmental events, light, and temperature in the expression of a clock gene. Generation of zebrafish that express the firefly luciferase gene under a clock gene promoter enables the study of molecular circadian rhythms in vivo
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影响因子：
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通讯作者：
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