Pre-existing T-cell immunity to SARS-CoV-2 in unexposed healthy controls in Ecuador, as detected with a COVID-19 Interferon-Gamma Release Assay.
Pre-existing T-cell immunity to SARS-CoV-2 in unexposed healthy controls in Ecuador, as detected with a COVID-19 Interferon-Gamma Release Assay.
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DOI：
10.1016/j.ijid.2021.02.034
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
de Waard JH
中科院分区：
文献类型：
--
作者：
Echeverría G;Guevara Á;Coloma J;Ruiz AM;Vasquez MM;Tejera E;de Waard JH
针对SARS-CoV-2的T细胞免疫应答的研究对于理解个体或群体的免疫状态非常重要。在这里，我们使用一种简单，廉价，快速的全血刺激试验-干扰素-γ释放试验（IGRA）-来研究恢复期COVID-19患者和厄瓜多尔基多未暴露的健康接触者对SARS-CoV-2的T细胞免疫力。分别用SARS-CoV-2刺突S1蛋白、受体结合结构域（RBD）蛋白或核衣壳（NP）蛋白刺激24 h后，在恢复期和未暴露受试者的肝素化血液中测量干扰素-γ（INF-γ）的产生。用“内部”ELISA测定两个研究组中IgG-RBD蛋白抗体的存在。根据INF-γ产生的测量，80%的恢复期COVID-19患者（全部为IgG-RBD血清阳性）具有强烈的T细胞应答。然而，出乎意料的是，44%的未暴露的健康对照（全部为IgG-RBD血清阴性）对COVID-19 IGRA产生了强烈的病毒特异性T细胞应答，这可能是因为之前暴露于常见的引起感冒的冠状病毒或其他病毒或微生物抗原。未暴露的健康受试者中具有预先存在的免疫力的比例很高，这表明厄瓜多尔部分人口可能具有SARS-CoV-2反应性T细胞。鉴于IGRA技术简单，可以很容易地扩大规模，用于需要大量患者的研究，这种COVID-19 IGRA可以用于确定在基于人群的研究中，仅T细胞反应是否代表对SARS-CoV-2感染的保护性免疫。
Studies of T-cell immune responses against SARS-CoV-2 are important in understanding the immune status of individuals or populations. Here, we use a simple, cheap, and rapid whole blood stimulation assay - an Interferon-Gamma Release Assay (IGRA) - to study T-cell immunity to SARS-CoV-2 in convalescent COVID-19 patients and in unexposed healthy contacts from Quito, Ecuador. Interferon-gamma (INF-γ) production was measured in the heparinized blood of convalescent and unexposed subjects after stimulation for 24 h with the SARS-CoV-2 Spike S1 protein, the Receptor Binding Domain (RBD) protein or the Nucleocapsid (NP) protein, respectively. The presence of IgG-RBD protein antibodies in both study groups was determined with an “in-house” ELISA. As measured with INF-γ production, 80% of the convalescent COVID-19 patients, all IgG-RBD seropositive, had a strong T-cell response. However, unexpectedly, 44% of unexposed healthy controls, all IgG-RBD seronegative, had a strong virus-specific T-cell response with the COVID-19 IGRA, probably because of prior exposure to common cold-causing coronaviruses or other viral or microbial antigens. The high percentage of unexposed healthy subjects with a pre-existing immunity suggests that a part of the Ecuadorian population is likely to have SARS-CoV-2 reactive T-cells. Given that the IGRA technique is simple and can be easily scaled up for investigations where high numbers of patients are needed, this COVID-19 IGRA may serve to determine if the T-cell only response represents protective immunity to SARS-CoV-2 infection in a population-based study.
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影响因子：
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作者：
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作者：
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DOI：
10.1016/j.cmi.2020.09.051
发表时间：
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期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
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作者：
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DOI：
10.1038/s41577-020-00460-4
发表时间：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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