A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis.
A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis.
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DOI：
10.1186/s12906-017-2002-y
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发表时间：
2017-11-22
影响因子：
--
通讯作者：
Waly NE
中科院分区：
文献类型：
--
作者：
Aborehab NM;El Bishbishy MH;Refaiy A;Waly NE
草药治疗可能对骨关节炎（OA）有软骨保护和治疗作用。研究了埃及产生姜和姜黄素根茎对OA大鼠模型的作用机制。35只白化病大鼠膝关节关节内注射碘乙酸钠。口服生姜和姜黄素，剂量分别为200和400 mg/kg （F200和F400）。ELISA法测定血清软骨寡聚基质蛋白（COMP）、透明质酸（HA）、丙二醛（MDA）、髓过氧化物酶（MPO）、白细胞介素-1β (IL-1β)和超氧化物歧化酶（SOD）水平。采用UPLC-ESI-MS对中药配方水乙醇提取物的组成进行了表征。采用常规组织病理学检查注射关节的组织病理学改变。统计学分析采用单因素方差分析。与OA组相比，f200、f400和V组血清COMP、HA、MPO、MDA、IL-1β水平均显著降低（P值<0.0001）。另一方面，与OA组相比，治疗组SOD水平显著升高（P值<0.0001）。姜/姜黄素1:1的比例通过抗炎和抗氧化作用对OA模型大鼠具有软骨保护作用。需要进一步的药理学和临床研究来评估这种效果。
Herbal treatment may have a chondroprotective and therapeutic effect on Osteoarthritis (OA). We investigated the mechanism of action of ginger and curcumin rhizomes cultivated in Egypt in treatment of OA in rat model. Thirty-five albino rats were intra-articularly injected with Monosodium Iodoacetate in the knee joint. Ginger and curcumin was orally administered at doses of 200 and 400 mg/kg (F200 and F400). Serum levels of cartilage oligomeric matrix protein (COMP), hyaluronic acid (HA), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin-1 beta (IL-1β) and superoxide dismutase activity (SOD) were measured using ELISA. The composition of the herbal formula hydro-ethanolic extract was characterized using UPLC-ESI-MS. Histopathological changes in injected joints was examined using routine histopathology. Statistical analysis was performed using one-way ANOVA. Serum levels of COMP, HA, MPO, MDA, and IL-1β were significantly decreased in F 200, F 400 and V groups when compared to OA group (P value <0.0001). On the other hand SOD levels were significantly elevated in treated groups compared to OA groups (P value <0.0001). The ginger/curcumin at 1:1 had chondroprotective effect via anti-inflammatory and antioxidant effect in rat OA model. Further pharmacological and clinical studies are needed to evaluate this effect.
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影响因子：
6.3
作者：
Ghavipour, Mahsa;Sotoudeh, Gity;Ghorbani, Mohammad
通讯作者：
Ghorbani, Mohammad
影响因子：
--
作者：
Hootman, JM;Helmick, CG
通讯作者：
Helmick, CG
影响因子：
6.1
作者：
Bianchi, Enrica;Mannelli, Lorenzo Di Cesare;Ghelardini, Carla
通讯作者：
Ghelardini, Carla
影响因子：
7
作者：
Hawker, G. A.;Mian, S.;Stanaitis, I.
通讯作者：
Stanaitis, I.
影响因子：
2
作者：
Jiang, HL;S贸lyom, AM;Gang, DR
通讯作者：
Gang, DR