L-rhamnose is often an important part of immunodominant epitope for pneumococcal serotype 23F polysaccharide antibodies in human sera immunized with PPV23.
L-rhamnose is often an important part of immunodominant epitope for pneumococcal serotype 23F polysaccharide antibodies in human sera immunized with PPV23.
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DOI：
10.1371/journal.pone.0083810
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nahm MH
中科院分区：
文献类型：
--
作者：
Park S;Nahm MH
肺炎链球菌是一种主要的人类病原体，其在表面表达90多种血清学上不同的荚膜多糖（PS）。由于肺炎链球菌PS会引发针对肺炎链球菌疾病的保护性抗体，因此确定引发抗肺炎链球菌PS抗体的免疫表位非常重要。L - 鼠李糖是23F PS重复单元的一部分，并且已知是引发针对肺炎链球菌23F血清型PS抗体的免疫显性表位的关键部分。为了确定L - 鼠李糖是否是人类血清样本中针对23F血清型PS的功能性抗体所识别的表位的一部分，我们评估了针对L - 鼠李糖的血清抗体对23F血清型肺炎链球菌的调理吞噬杀伤作用。使用10 mM L - 鼠李糖，在53份接种23价肺炎链球菌多糖疫苗（PPV23）的年轻人和老年人血清中，19份血清（36%）的血清抗体调理能力（调理指数）被抑制60%，16份血清（30%）被抑制30 - 60%。有趣的是，当从免疫血清中去除IgM抗体以便优先研究IgG抗体时，10 mM L - 鼠李糖对调理能力抑制超过60%的年轻人血清比例从33%（11/31）增加到68%（21/31）。另一方面，IgM去除并没有改变老年人血清的比例。因此，年轻人和老年人可能针对23F血清型PS产生不同的IgG抗体抗原结合谱。
Streptococcus pneumoniae is a major human pathogen which expresses more than 90 serologically distinct capsular polysaccharides (PS) on the surface. Since pneumococcal PSs elicit protective antibodies against pneumococcal diseases, it is important to identify the immunological epitope eliciting anti-pneumococcal PS antibodies. L-rhamnose is a part of the 23F PS repeating unit and is known to be a critical part of immunodominant epitope which elicits antibodies against pneumococcal serotype 23F PS. In order to determine if L-rhamnose is a part of epitope recognized by functional antibodies specific for serotype 23F PS in human serum samples, we evaluated the opsonophagocytic killing of serotype 23F pneumococci by serum antibodies specific for L-rhamnose. Using 10 mM L-rhamnose, opsonic capacities (opsonic indices) of serum antibodies were inhibited by 60% in 19 sera (36%) and 30–60% in 16 sera (30%) out of 53 sera from young and old adults immunized with 23-valent pneumococcal polysaccharide vaccine (PPV23). Interestingly, when IgM antibodies were depleted from immune sera in order to preferentially study IgG antibodies, the proportion of young adult sera showing more than 60% inhibition in opsonic capacity by 10 mM of L-rhamnose increased from 33% (11/31) to 68% (21/31). On the other hand, IgM depletion did not alter the proportion for old adult sera. Therefore, young and old adults may produce different antigen binding profiles of IgG antibodies against serotype 23F PS.
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DOI：
10.1084/jem.142.2.435
发表时间：
1975-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cisar J;Kabat EA;Dorner MM;Liao J
通讯作者：
Liao J
影响因子：
6.4
作者：
Leggat, David J.;Thompson, Rebecca S.;Westerink, M. A. Julie
通讯作者：
Westerink, M. A. Julie
影响因子：
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作者：
Burton, Robert L.;Nahm, Moon H.
通讯作者：
Nahm, Moon H.
影响因子：
5.5
作者：
Jackson, Lisa A.;Neuzil, Kathleen M.;Carlone, George M.
通讯作者：
Carlone, George M.
DOI：
10.1084/jem.54.1.73
发表时间：
1931-06-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Avery OT;Dubos R
通讯作者：
Dubos R