Short-term sleep deprivation stimulates hippocampal neurogenesis in rats following global cerebral ischemia/reperfusion.
Short-term sleep deprivation stimulates hippocampal neurogenesis in rats following global cerebral ischemia/reperfusion.
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短期睡眠剥夺刺激大鼠全脑缺血/再灌注后海马神经发生
DOI：
10.1371/journal.pone.0125877
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Cheng O;Li R;Zhao L;Yu L;Yang B;Wang J;Chen B;Yang J
背景睡眠剥夺在中枢神经系统疾病中起着复杂的作用。最近的研究表明，短期SD可以影响缺血损伤的程度。本研究旨在探讨短期给药能否刺激全脑缺血再灌流(GCIR)大鼠海马神经细胞的再生。方法100只SD大鼠随机分为假手术组、GCIR组和短期SD组，短期SD组又随机分为GCIR+6hSD*3d组、GCIR+12hSD组和GCIR+12hSD*3d组。采用双侧颈总动脉结扎加失血性低血压的方法建立大鼠GCIR模型。大鼠在GCIR后48小时开始睡眠剥夺。用Morris水迷宫测试大鼠的学习记忆能力，用5-溴脱氧尿苷(BrdU)和神经元特异性烯醇化酶(NSE)分别在第14和28d检测细胞增殖和分化，7d后检测海马区BDNF的表达。此外，与GCIR组相比，短期SD组的BrdU-和BrdU/NSE阳性细胞数也有所增加。在三个短期SD组中，GCIR+12hSD*3D治疗组的益处最大。结论短期SD，尤其是GCIR+12hSD*3D治疗方法，可促进GCIR大鼠海马齿状回(DG)神经发生，BDNF可能是其作用机制之一。
Background Sleep deprivation (SD) plays a complex role in central nervous system (CNS) diseases. Recent studies indicate that short-term SD can affect the extent of ischemic damage. The aim of this study was to investigate whether short-term SD could stimulate hippocampal neurogenesis in a rat model of global cerebral ischemia/reperfusion (GCIR). Methods One hundred Sprague-Dawley rats were randomly divided into Sham, GCIR and short-term SD groups based on different durations of SD; the short-term SD group was randomly divided into three subgroups: the GCIR+6hSD*3d-treated, GCIR+12hSD-treated and GCIR+12hSD*3d-treated groups. The GCIR rat model was induced via the bilateral occlusion of the common carotid arteries and hemorrhagic hypotension. The rats were sleep-deprived starting at 48 h following GCIR. A Morris water maze test was used to assess learning and memory ability; cell proliferation and differentiation were analyzed via 5-bromodeoxyuridine (BrdU) and neuron-specific enolase (NSE), respectively, at 14 and 28 d; the expression of hippocampal BDNF was measured after 7 d. Results The different durations of short-term SD designed in our experiment exhibited improvement in cognitive function as well as increased hippocampal BDNF expression. Additionally, the short-term SD groups also showed an increased number of BrdU- and BrdU/NSE-positive cells compared with the GCIR group. Of the three short-term SD groups, the GCIR+12hSD*3d-treated group experienced the most substantial beneficial effects. Conclusions Short-term SD, especially the GCIR+12hSD*3d-treated method, stimulates neurogenesis in the hippocampal dentate gyrus (DG) of rats that undergo GCIR, and BDNF may be an underlying mechanism in this process.
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黄芩苷通过减少海马细胞凋亡提高整体缺血/再灌注大鼠的空间学习能力
影响因子：
2.9
作者：
Cheng, Oumei;Li, Zhenhua;Cheng, Ke
通讯作者：
Cheng, Ke
DOI：
10.1155/2011/654085
发表时间：
2011
期刊：
International journal of peptides
影响因子：
--
作者：
Murray PS;Holmes PV
通讯作者：
Holmes PV
影响因子：
2.9
作者：
Kuang G;He Q;Zhang Y;Zhuang R;Xiang A;Jiang Q;Luo Y;Yang J
通讯作者：
Yang J
DOI：
10.1073/pnas.0608644103
发表时间：
2006-12-12
影响因子：
11.1
作者：
Mirescu, Christian;Peters, Jennifer D.;Gould, Elizabeth
通讯作者：
Gould, Elizabeth
影响因子：
2.5
作者：
Hairston, IS;Peyron, C;O'Hara, BF
通讯作者：
O'Hara, BF