A Transcriptional Sequencing Analysis of Islet Stellate Cell and Pancreatic Stellate Cell.
A Transcriptional Sequencing Analysis of Islet Stellate Cell and Pancreatic Stellate Cell.
复制标题
DOI：
10.1155/2018/7361684
复制
发表时间：
2018
影响因子：
4.3
通讯作者：
Sun Z
中科院分区：
文献类型：
--
作者：
Wang X;Li W;Chen J;Zhao S;Qiu S;Yin H;Carvalho V;Zhou Y;Shi R;Hu J;Li S;Nijiati M;Sun Z
我们前期的研究表明，胰岛星状细胞（islet stellate cell，ISC）与胰腺星状细胞（pancreatic stellate cell，PSC）在表型和生物学特性上相似，可能是2型糖尿病胰岛纤维化的主要原因。为了进一步确定PSC和ISC之间的差异，并更好地了解ISC的生理功能，我们对Wistar大鼠的PSC和ISC进行了全基因组转录分析。 在相同的条件下原代培养来自每只大鼠的PSC和ISCs。产生星状细胞的全基因组转录序列。用RT-PCR方法对差异表达基因进行验证。 共筛选出32个差异表达基因。此外，发现11 a1型胶原（COL 11 A1）在ISC中的表达是PSC的2.91倍，表明COL 11 A1可能是调节PSC和ISC之间差异的潜在关键基因。 我们的研究确定并验证了PSC和ISC在全基因组转录水平上的差异，证实了ISC和PSC相似而非相同的假设。此外，我们的数据可能有助于进一步研究ISC和胰岛纤维化，一些差异表达的基因可能为2型糖尿病提供新的治疗靶点。
Our previous studies have shown that islet stellate cell (ISC), similar to pancreatic stellate cell (PSC) in phenotype and biological characters, may be responsible for the islet fibrosis in type 2 diabetes. To further identify the differences between PSC and ISC and for better understanding of the physiological function of ISC, we employed genome-wide transcriptional analysis on the PSCs and ISCs of Wistar rats. PSCs and ISCs from each rat were primarily cultured at the same condition. Genome-wide transcriptional sequence of stellate cells was generated. The identified differentially expressed genes were validated using RT-PCR. 32 significant differentially expressed genes between PSCs and ISCs were identified. Moreover, collagen type 11a1 (COL11A1), was found to be expressed 2.91-fold higher in ISCs compared with PSCs, indicating that COL11A1 might be a potential key gene modulating the differences between PSC and ISC. Our study identified and validated the differences between PSC and ISC in genome-wide transcriptional scale, confirming the assumption that ISC and PSC are similar other than identical. Moreover, our data might be instrumental for further investigation of ISC and islet fibrosis, and some differential expressed genes may provide an insight into new therapeutic targets for type 2 diabetes.
登录
查看更多内容
影响因子：
4.3
作者：
Li FF;Chen BJ;Li W;Li L;Zha M;Zhou S;Bachem MG;Sun ZL
通讯作者：
Sun ZL
DOI：
10.1016/j.bbrc.2011.09.087
发表时间：
2011-10-22
影响因子：
3.1
作者：
Lee, Esder;Ryu, Gyeong Ryul;Song, Ki-Ho
通讯作者：
Song, Ki-Ho
影响因子：
7.7
作者：
Chiasson, JL;Rabasa-Lhoret, M
通讯作者：
Rabasa-Lhoret, M
影响因子：
--
作者：
Schmalbach, CE;Chepeha, DB;Hanash, S
通讯作者：
Hanash, S
影响因子：
5.4
作者：
Zha, M.;Zhang, M.;Yang, T.
通讯作者：
Yang, T.