Human umbilical cord blood mononuclear cell transplantation in rats with intrinsic sphincter deficiency.
Human umbilical cord blood mononuclear cell transplantation in rats with intrinsic sphincter deficiency.
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DOI：
10.3346/jkms.2010.25.5.663
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发表时间：
2010-05
影响因子：
4.5
通讯作者：
Lee KJ
中科院分区：
文献类型：
--
作者：
Lim JJ;Jang JB;Kim JY;Moon SH;Lee CN;Lee KJ
为了评估人脐带血（HUCB）移植治疗内括约肌缺陷（ISD）的有效性，我们分析了 HUCB 单核细胞移植对诱导性 ISD 大鼠的短期效果。 1周后注射HUCB单核细胞电烧尿道周围软组织，诱导大鼠ISD。 4周时，与对照组相比，实验组通过平均漏点压力测量的括约肌功能显着改善。 （91.75±18.99 mmHg 与 65.02±22.09 mmHg，P=0.001）。组织学上，括约肌恢复而没有损伤，而在对照组中，括约肌出现明显破坏，出现萎缩的肌肉层和胶原沉积。我们通过 Di-I 信号和普鲁士蓝染色鉴定了组织切片中注射的 HUCB 细胞。 HUCB单核细胞注射显着改善尿道括约肌功能，提示其治疗ISD的潜在功效。
To evaluate the effectiveness of the human umbilical cord blood (HUCB) transplantation for the treatment of intrinsic sphincter deficiency (ISD), we analyzed the short term effects of HUCB mononuclear cell transplantation in rats with induced-ISD. ISD was induced in rats by electro-cauterization of periurethral soft tissue with HUCB mononuclear cell injection after 1 week. The sphincter function measured by mean leak point pressure was significantly improved in the experimental group compared to the control group at 4 weeks. (91.75±18.99 mmHg vs. 65.02±22.09 mmHg, P=0.001). Histologically, the sphincter muscle was restored without damage while in the control group it appeared markedly disrupted with atrophic muscle layers and collagen deposit. We identified injected HUCB cells in the tissue sections by Di-I signal and Prussian blue staining. HUCB mononuclear cell injection significantly improved urethral sphincter function, suggesting its potential efficacy in the treatment of ISD.
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