Bactericidal activity and recovery effect of hydroxyl radicals generated by ultraviolet irradiation and silver ion application on an infected titanium surface
Bactericidal activity and recovery effect of hydroxyl radicals generated by ultraviolet irradiation and silver ion application on an infected titanium surface
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紫外线照射和银离子涂抹产生的羟基自由基对受感染钛表面的杀菌活性和恢复效果
DOI：
10.1038/s41598-020-65411-4
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发表时间：
2020
影响因子：
4.6
通讯作者：
Sasaki K.
中科院分区：
文献类型：
--
作者：
Tenkumo T;Ishiyama K;Prymak O;Nakamura K;Shirato M;Ogawa T;Miyashita M;Takahashi M;Epple M;Kanno T;Sasaki K.
本研究采用银离子应用和紫外-A (UV-A) 光照射联合处理，研究了杀菌效果、治疗的基本机制以及受感染钛表面生物相容性的恢复。变形链球菌和放线菌聚集菌以悬浮液形式使用，并作为钛表面上的生物膜来测试杀菌效果。联合处理的杀菌效果明显高于单独施用银离子或UV-A光照射。联合处理的杀菌作用归因于细菌细胞壁产生的羟基自由基，其产量随着银浓度的增加而增加。为了评估生物相容性，在处理过的钛表面上评估了 MC3T3E1 细胞的增殖和钙化。将经过处理的钛螺钉植入大鼠胫骨，并在术后 28 天测量移除扭矩。经过联合处理的钛表面通过允许细胞增殖或钙化达到在未感染的钛表面中观察到的水平而表现出生物相容性的恢复。术后 28 天的移除扭矩也与对照值相当。这种方法是种植体周围炎的一种新颖的治疗选择。
This study investigated the bactericidal effect, the underlying mechanisms of treatment, and recovery of biocompatibility of the infected titanium surface using a combination treatment of silver ion application and ultraviolet-A (UV-A) light irradiation.Streptococcus mutansandAggregatibacter actinomycetemcomitanswere used in suspension and as a biofilm on a titanium surface to test for the bactericidal effect. The bactericidal effect of the combination treatment was significantly higher than that of silver ion application or UV-A light irradiation alone. The bactericidal effect of the combination treatment was attributable to hydroxyl radicals, which generated from the bacterial cell wall and whose yield increased with the silver concentration. To assess the biocompatibility, proliferation and calcification of MC3T3E1 cells were evaluated on the treated titanium surface. The treated titanium screws were implanted into rat tibias and the removal torques were measured 28 days post-surgery. The titanium surface that underwent the combination treatment exhibited recovery of biocompatibility by allowing cellular proliferation or calcification at levels observed in the non-infected titanium surfaces. The removal torque 28 days after surgery was also comparable to the control values. This approach is a novel treatment option for peri-implantitis.
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影响因子：
3.7
作者：
Sheng H;Nakamura K;Kanno T;Sasaki K;Niwano Y
通讯作者：
Niwano Y
不同糖类对乳酸菌原生质体形成的影响
DOI：
10.4044/joma1947.96.7-8_757
发表时间：
1984
期刊：
Okayama Igakkai Zasshi (journal of Okayama Medical Association)
影响因子：
--
作者：
Akira Kumode
通讯作者：
Akira Kumode
影响因子：
2
作者：
Ogawa, Takahiro
通讯作者：
Ogawa, Takahiro
空气粉末研磨处理作为种植体表面清洁方法：文献综述。
DOI：
--
发表时间：
2012
期刊：
The International Journal of Oral and Maxillofacial Implants
影响因子：
--
作者：
C. S. Tastepe;R. van Waas;Y. Liu;D. Wismeijer
通讯作者：
D. Wismeijer
通过 Vector 系统治疗种植体周围炎。
影响因子：
4.3
作者：
E. Karring;A. Stavropoulos;B. Ellegaard;T. Karring
通讯作者：
T. Karring