Impact of ileocecal resection and concomitant antibiotics on the microbiome of the murine jejunum and colon.
Impact of ileocecal resection and concomitant antibiotics on the microbiome of the murine jejunum and colon.
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DOI：
10.1371/journal.pone.0073140
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Azcarate-Peril MA
中科院分区：
文献类型：
--
作者：
Devine AA;Gonzalez A;Speck KE;Knight R;Helmrath M;Lund PK;Azcarate-Peril MA
回盲部切除术（ICR）是一种常见的需要在难以管理的克罗恩病和坏死性小肠结肠炎的手术干预。然而，ICR以及与ICR常规给予的抗生素伴随剂量对肠道肠道菌群的影响尚未确定。在本研究中，对野生型C57 BL 6小鼠进行ICR和伴随的单次腹膜内抗生素注射。在切除后7、14和28天从空肠和结肠收集肠腔内容物，并与非ICR对照组进行比较。采用16 S rRNA基因焦磷酸测序和定量PCR技术对样本进行分析。在ICR和伴随的抗生素治疗后7天，肠道微生物群发生了改变，结肠中的多样性降低。系统发育多样性（PD）从非ICR对照组的11.8 ± 1.8降至ICR后7天样本的5.9 ± 0.5。空肠中也存在轻微影响，PD值从8.3 ± 0.4降至7.5 ± 1.4。PCoA分析表明，ICR后28天的细菌种群与非ICR对照组显着不同。此外，结肠和空肠的细菌种群是非常相似的切除后28天，而最初的社区显着不同。在ICR前，厚壁菌门和拟杆菌门是空肠和结肠的优势门;然而，在ICR后28天，厚壁菌门成为空肠和结肠的极大优势门。尽管微生物群恢复到稳态，厚壁菌门重新成为主要门，但我们在ICR后28天未在结肠中检测到拟杆菌门。在该时间段后，在空肠中检测到0.01%丰度的拟杆菌。因此，ICR和伴随的抗生素注射诱导的空肠和结肠微生物群的变化可被认为是手术后适应性生长或功能的潜在调节剂，并且在活动性IBD的情况下，是疾病复发的手术后病理生理学的潜在贡献者。
Ileocecal resection (ICR) is a commonly required surgical intervention in unmanageable Crohn’s disease and necrotizing enterocolitis. However, the impact of ICR, and the concomitant doses of antibiotic routinely given with ICR, on the intestinal commensal microbiota has not been determined. In this study, wild-type C57BL6 mice were subjected to ICR and concomitant single intraperitoneal antibiotic injection. Intestinal lumen contents were collected from jejunum and colon at 7, 14, and 28 days after resection and compared to non-ICR controls. Samples were analyzed by16S rRNA gene pyrosequencing and quantitative PCR. The intestinal microbiota was altered by 7 days after ICR and accompanying antibiotic treatment, with decreased diversity in the colon. Phylogenetic diversity (PD) decreased from 11.8 ± 1.8 in non-ICR controls to 5.9 ± 0.5 in 7-day post-ICR samples. There were also minor effects in the jejunum where PD values decreased from 8.3 ± 0.4 to 7.5 ± 1.4. PCoA analysis indicated that bacterial populations 28 days post-ICR differed significantly from non-ICR controls. Moreover, colon and jejunum bacterial populations were remarkably similar 28 days after resection, whereas the initial communities differed markedly. Firmicutes and Bacteroidetes were the predominant phyla in jejunum and colon before ICR; however, Firmicutes became the vastly predominant phylum in jejunum and colon 28 days after ICR. Although the microbiota returned towards a homeostatic state, with re-establishment of Firmicutes as the predominant phylum, we did not detect Bacteroidetes in the colon 28 days after ICR. In the jejunum Bacteroidetes was detected at a 0.01% abundance after this time period. The changes in jejunal and colonic microbiota induced by ICR and concomitant antibiotic injection may therefore be considered as potential regulators of post-surgical adaptive growth or function, and in a setting of active IBD, potential contributors to post-surgical pathophysiology of disease recurrence.
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影响因子：
24.5
作者：
Korzenik, J. R.
通讯作者：
Korzenik, J. R.
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
2.4
作者：
Klaus, Jochen;Spaniol, Ulrike;von Tirpitz, Christian C.
通讯作者：
von Tirpitz, Christian C.
影响因子：
4.3
作者：
Bures, Jan;Cyrany, Jiri;Kopacova, Marcela
通讯作者：
Kopacova, Marcela
影响因子：
--
作者：
Cole, Conrad R;Ziegler, Thomas R
通讯作者：
Ziegler, Thomas R