Evaluation of inflammatory response to acute ischemia using near-infrared fluorescent reactive oxygen sensors.
Evaluation of inflammatory response to acute ischemia using near-infrared fluorescent reactive oxygen sensors.
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DOI：
10.1007/s11307-013-0614-2
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发表时间：
2013-08
影响因子：
3.1
通讯作者：
Akers, Walter J.
中科院分区：
文献类型：
--
作者：
Magalotti, Selena;Gustafson, Tiffany P.;Cao, Qian;Abendschein, Dana R.;Pierce, Richard A.;Berezin, Mikhail Y.;Akers, Walter J.
与炎症分子（如活性氧（ROS））产生相关的缺血相关过程在出现组织损伤的生理和解剖证据之前难以在急性期检测到。体内缺血事件后早期评估炎症和愈合反应将有助于治疗选择和患者预后。我们介绍了一种新的近红外氢菁分子探针，用于检测ROS作为组织对缺血反应的标记和血管生成和重塑的前兆。合成的分子探针，最初是与聚乙二醇偶联的非荧光氢菁，在氧化后转化为高荧光菁报告蛋白。该探针应用于临床前小鼠心肌梗死模型，结扎和切除后肢部分股动脉导致暂时性缺血，随后血管生成和愈合。在24小时内，与未损伤的对照组肢体相比，缺血组织中观察到的荧光强度增加了约6倍，这是由于缺血组织中ROS浓度较高。这些结果表明，在询问缺血组织的炎症和愈合反应的非侵入性传感的潜力。
Ischemia-related processes associated with generation of inflammatory molecules such as reactive oxygen species (ROS) are difficult to detect at the acute stage before physiologic and anatomic evidence of tissue damage is present. Evaluation of the inflammatory and healing response early after an ischemic event in vivo will aid in treatment selection and patient outcomes. We introduce a novel near-infrared hydrocyanine molecular probe for detection of ROS as a marker of tissue response to ischemia and a precursor to angiogenesis and remodeling. The synthesized molecular probe, initially a non-fluorescent hydrocyanine conjugated to polyethyleneglycol, converts to a highly fluorescent cyanine reporter upon oxidation. The probe was applied in a preclinical mouse model for myocardial infarction, where ligation and removal of a portion of the femoral artery in the hindlimb resulted in temporary ischemia followed by angiogenesis and healing. The observed increase in fluorescence intensity was approximately 6-fold over 24 h in the ischemic tissue relative to the uninjured control limb and was attributed to the higher concentration of ROS in the ischemic tissue. These results demonstrate the potential for non-invasive sensing for interrogating the inflammatory and healing response in ischemic tissue.
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影响因子：
16.6
作者：
Kundu, Kousik;Knight, Sarah F.;Lee, Seungjun;Taylor, W. Robert;Murthy, Niren
通讯作者：
Murthy, Niren
影响因子：
3.8
作者：
Liu, Yang;Bauer, Adam Q.;Akers, Walter J.;Sudlow, Gail;Liang, Kexian;Shen, Duanwen;Berezin, Mikhail Y.;Culver, Joseph P.;Achilefu, Samuel
通讯作者：
Achilefu, Samuel
影响因子：
--
作者：
Gustafson TP;Yan Y;Newton P;Hunter DA;Achilefu S;Akers WJ;Mackinnon SE;Johnson PJ;Berezin MY
通讯作者：
Berezin MY
影响因子：
14
作者：
Selvam, Shivaram;Kundu, Kousik;Templeman, Kellie L.;Murthy, Niren;Garcia, Andres J.
通讯作者：
Garcia, Andres J.
DOI：
10.1164/rccm.2206007
发表时间：
2002-12-15
影响因子：
24.7
作者：
Forman, HJ;Torres, M
通讯作者：
Torres, M