Multifunctional biodegradable polyacrylamide nanocarriers for cancer theranostics--a "see and treat" strategy.
Multifunctional biodegradable polyacrylamide nanocarriers for cancer theranostics--a "see and treat" strategy.
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DOI：
10.1021/nn301633m
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发表时间：
2012-08-28
期刊：
影响因子：
17.1
通讯作者：
Kopelman, Raoul
中科院分区：
文献类型：
--
作者：
Wang, Shouyan;Kim, Gwangseong;Lee, Yong-Eun Koo;Hah, Hoe Jin;Ethirajan, Manivannan;Pandey, Ravindra K.;Kopelman, Raoul
我们在此描述了基于胺功能化可生物降解的聚丙烯酰胺纳米颗粒（NPs）的多功能纳米载体的发展，用于癌症治疗，包括主动肿瘤靶向，荧光成像和光动力治疗。结构设计包括在NP聚合过程中添加一级氨基和可生物降解的交联剂，同时在NP基质中加入光动力和荧光显像剂，并将PEG和肿瘤靶向配体偶联到NP表面。合成的纳米粒子为球形，平均直径为44 nm。一项使用氢氧化钠或猪肝酯酶的加速生物降解研究表明，水凝胶聚合物基质链在几天内就会崩溃。用凝胶渗透色谱法检测降解后的小分子。对人乳腺癌细胞的体外靶向研究表明，靶向的NPs可以有效地转运到肿瘤细胞中。将多功能纳米载体培养到癌细胞中可以实现强荧光成像。结合在NPs内的光敏药物，用合适波长的光照射，对浸渍的肿瘤细胞造成显著但选择性的损伤，但仅在照射区域内。总的来说，聚合物基NPs作为可生物降解的多功能纳米载体的潜力，在癌症治疗中得到了证明。
We describe here the development of multifunctional nanocarriers, based on amine functionalized biodegradable polyacrylamide nanoparticles (NPs), for cancer theranostics, including active tumor targeting, fluorescence imaging and photodynamic therapy. The structural design involves adding primary amino groups and biodegradable crosslinkers during the NP polymerization, while incorporating photodynamic and fluorescent imaging agents into the NP matrix, and conjugating PEG and tumor-targeting ligands onto the surface of the NPs. The as-synthesized NPs are spherical, with an average diameter of 44 nm. An accelerated biodegradation study, using sodium hydroxide or porcine liver esterase, indicated a hydrogel polymer matrix chain collapse within several days. By using gel permeation chromatography, small molecules were detected, after the degradation. In vitro targeting studies on human breast cancer cells indicate that the targeted NPs can be transported efficiently into tumor cells. Incubating the multifunctional nanocarriers into cancer cells enabled strong fluorescence imaging. Irradiation of the photosensitizing drug, incorporated within the NPs, with light of a suitable wavelength, causes significant but selective damage to the impregnated tumor cells, but only inside the illuminated areas. Overall, the potential of polymeric-based NPs as biodegradable, multifunctional nanocarriers, for cancer theranostics, is demonstrated here.
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影响因子：
5.4
作者：
Gupta, Anurag;Wang, Shouyan;Pera, Paula;Rao, K. V. R.;Patel, Nayan;Ohulchanskyy, Tymish Y.;Missert, Joseph;Morgan, Janet;Koo-Lee, Yong-Eun;Kopelman, Raoul;Pandey, Ravindra K.
通讯作者：
Pandey, Ravindra K.
影响因子：
2.7
作者：
Kopelman, R;Koo, YEL;Ross, BD
通讯作者：
Ross, BD
影响因子：
4.9
作者：
Baba, Koichi;Pudavar, Haridas E.;Prasad, Paras N.
通讯作者：
Prasad, Paras N.
影响因子：
10.8
作者：
Fonseca, C;Simoes, S;Gaspar, R
通讯作者：
Gaspar, R
影响因子：
10.8
作者：
Gao D;Xu H;Philbert MA;Kopelman R
通讯作者：
Kopelman R