Hematopoiesis at the onset of metamorphosis: terminal differentiation and dissociation of the Drosophila lymph gland.
Hematopoiesis at the onset of metamorphosis: terminal differentiation and dissociation of the Drosophila lymph gland.
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DOI：
10.1007/s00427-011-0364-6
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发表时间：
2011-08
影响因子：
2.4
通讯作者：
Hartenstein V
中科院分区：
文献类型：
--
作者：
Grigorian M;Mandal L;Hartenstein V
黑腹果蝇的造血器官（称为淋巴腺）在整个幼虫期增殖和分化。晚期幼虫的淋巴腺由一个大的初级叶和几个较小的次级叶组成。分化为两种类型的血细胞：浆细胞和晶体细胞，仅限于初级叶的外层（皮质区）；初级叶（髓质区）的中心以及次级叶仅包含增殖的原血细胞。位于初级叶后尖的一小群原血细胞充当信号中心（PSC），抑制髓质区的早熟分化。幼虫淋巴腺由包围单个血细胞、血细胞群以及整个淋巴腺的细胞外基质层（基底膜）稳定。在本文中，我们研究了早期蛹中淋巴腺的塑造事件。在蛹形成 (APF) 后的前 12 小时内，淋巴腺解离，血细胞分散，留下基底膜的空壳。在淋巴腺解离之前，髓质区的细胞分化，表达早期分化标记物过氧化物酶，以及部分表达晚期分化标记物 P1。 PSC 细胞在扩散之前遍布整个蛹淋巴腺。次生叶细胞经历快速增殖期，持续到 APF 8 小时，然后表达过氧化物酶并分散。这些血细胞不表达 P1，表明它们在完全成熟之前就分散了。
The D. melanogaster hematopoietic organ, called lymph gland, proliferates and differentiates throughout the larval period. The lymph gland of the late larva is comprised of a large primary lobe and several smaller secondary lobes. Differentiation into two types of hemocytes, plasmatocytes and crystal cells, is confined to the outer layer (cortical zone) of the primary lobe; the center of the primary lobe (medullary zone), as well as the secondary lobes, contain only proliferating prohemocytes. A small cluster of prohemocytes located at the posterior tip of the primary lobe serves as a signaling center (PSC) that inhibits precocious differentiation of the medullary zone. The larval lymph gland is stabilized by layers of extracellular matrix (basement membranes) that surround individual hemocytes, groups of hemocytes, as well as the lymph gland as a whole. In this paper we investigated the events shaping the lymph gland in the early pupa. The lymph gland dissociates and hemocytes disperse during the first 12h after puparium formation (APF), leaving behind empty husks of basement membrane. Prior to lymph gland dissociation, cells of the medullary zone differentiate, expressing the early differentiation marker Peroxidasin, as well as, in part, the late differentiation marker P1. Cells of the PSC spread out throughout the pupal lymph gland prior to their dispersal. Cells of the secondary lobes undergo a rapid phase of proliferation that lasts until 8h APF, followed by expression of Peroxidasin, and dispersal. These hemocytes do not express P1, indicating that they disperse prior to full maturation.
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影响因子：
0.9
作者：
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通讯作者：
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