C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells.
C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells.
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C 型利钠肽/利钠肽受体 2 参与小鼠间质细胞的细胞增殖和睾酮生成
DOI：
10.5534/wjmh.180041
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发表时间：
2019-05
期刊：
影响因子：
--
通讯作者：
Huang S
中科院分区：
文献类型：
--
作者：
Yang L;Lei L;Zhao Q;Gong Y;Guan G;Huang S
目的 本研究探讨了利钠肽受体 2 (NPR2) 对小鼠 Leydig 细胞增殖和睾酮分泌的作用。材料与方法分离出生后不同阶段的小鼠睾丸，采用定量逆转录聚合酶链反应（RT-qPCR）检测C型利钠肽（CNP）及其受体NPR2的表达。培养从小鼠睾丸分离的 Leydig 细胞并用 shNPR2 慢病毒或 CNP 处理。然后通过ELISA、RT-qPCR、Cell Counting Kit-8和流式细胞术分析小鼠Leydig细胞中环磷酸鸟苷的产生、睾酮分泌、细胞增殖、细胞周期和细胞凋亡。 RT-qPCR、Western blot检测NPR2、细胞周期、凋亡增殖及细胞周期相关基因的表达。结果 RNAi 敲低 NPR2 导致小鼠 Leydig 细胞 S 期细胞周期停滞、细胞凋亡和睾酮分泌减少。结论本研究为更好地了解CNP/NPR2通路在男性生殖中的功能提供了更多证据，有助于我们治疗男性不育症。
Purpose This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secretion in mouse Leydig cells. Materials and Methods Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. And then the cyclic guanosine monophosphate production, testosterone secretion, cell proliferation, cell cycle and cell apoptosis in mouse Leydig cells were analyzed by ELISA, RT-qPCR, Cell Counting Kit-8, and flow cytometry. Moreover, the expression of NPR2, cell cycle, apoptosis proliferation and cell cycle related gene were detected by RT-qPCR and Western blot. Results Knockdown of NPR2 by RNAi resulted in S phase cell cycle arrest, cell apoptosis, and decreased testosterone secretion in mouse Leydig cells. Conclusions Our study provides more evidences to better understand the function of CNP/NPR2 pathway in male reproduction, which may help us to treat male infertility.
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影响因子：
3.7
作者：
Zhang J;Wei Q;Cai J;Zhao X;Ma B
通讯作者：
Ma B
影响因子：
4.6
作者：
Kong N;Xu X;Zhang Y;Wang Y;Hao X;Zhao Y;Qiao J;Xia G;Zhang M
通讯作者：
Zhang M
影响因子：
4
作者：
Yang L;Wei Q;Li W;Xi Q;Zhao X;Ma B
通讯作者：
Ma B
影响因子：
2.9
作者：
Xia H;Chen Y;Wu KJ;Zhao H;Xiong CL;Huang DH
通讯作者：
Huang DH
DOI：
10.1016/0006-291x(91)90627-j
发表时间：
1991-06-28
影响因子：
3.1
作者：
FURUYA, M;YOSHIDA, M;MATSUO, H
通讯作者：
MATSUO, H