Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering.
Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering.
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DOI：
10.1016/j.biomaterials.2011.04.061
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发表时间：
2011-09
期刊：
影响因子：
14
通讯作者：
Kundu, Subhas C.
中科院分区：
文献类型：
--
作者：
Bhardwaj, Nandana;Nguyen, Quynhhoa T.;Chen, Albert C.;Kaplan, David L.;Sah, Robert L.;Kundu, Subhas C.
利用细胞-支架构建体是一种很有前途的组织工程方法来修复软骨缺损和研究软骨组织的形成。本研究采用丝素蛋白/壳聚糖共混材料构建软骨组织工程支架。丝素蛋白作为细胞粘附和增殖的基质，而壳聚糖具有类似于糖胺聚糖的结构，并显示出软骨修复的前景。我们比较了丝素蛋白/壳聚糖共混支架与牛软骨细胞接种并体外培养2周后软骨组织的形成情况。对构建体进行细胞活力、组织学、细胞外基质成分糖胺聚糖和I型和II型胶原蛋白以及生物力学特性分析。丝素蛋白/壳聚糖支架支持细胞附着和生长，以及由阿尔新蓝组织化学和II型与I型胶原的相对表达所指示的软骨形成表型。糖胺聚糖和胶原在所有支架中积累，在丝素蛋白/壳聚糖（1：1）混合支架中最高。细胞接种结构在高频下的静态和动态刚度高于非接种对照。结果表明，丝/壳聚糖支架可能是一个有用的替代合成细胞支架软骨组织工程。
The use of cell-scaffold constructs is a promising tissue engineering approach to repair cartilage defects and to study cartilaginous tissue formation. In this study, silk fibroin/chitosan blended scaffolds were fabricated and studied for cartilage tissue engineering. Silk fibroin served as a substrate for cell adhesion and proliferation while chitosan has a structure similar to that of glycosaminoglycans, and shows promise for cartilage repair. We compared the formation of cartilaginous tissue in silk fibroin/chitosan blended scaffolds seeded with bovine chondrocytes and cultured in vitro for 2 weeks. The constructs were analyzed for cell viability, histology, extracellular matrix components glycosaminoglycan and collagen types I and II, and biomechanical properties. Silk fibroin/chitosan scaffolds supported cell attachment and growth, and chondrogenic phenotype as indicated by Alcian Blue histochemistry and relative expression of type II versus type I collagen. Glycosaminoglycan and collagen accumulated in all the scaffolds and was highest in the silk fibroin/chitosan (1:1) blended scaffolds. Static and dynamic stiffness at high frequencies was higher in cell-seeded constructs than non-seeded controls. The results suggest that silk/chitosan scaffolds may be a useful alternative to synthetic cell scaffolds for cartilage tissue engineering.
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影响因子：
14
作者：
Chen, Yen-Lin;Lee, Hsiao-Ping;Hu, Yu-Chen
通讯作者：
Hu, Yu-Chen
影响因子：
14
作者：
Chang, CH;Liu, HC;Lin, FH
通讯作者：
Lin, FH
影响因子：
7
作者：
Hoemann, CD;Sun, J;Buschmann, MD
通讯作者：
Buschmann, MD
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
9.7
作者：
Alves da Silva, M. L.;Crawford, A.;Neves, N. M.
通讯作者：
Neves, N. M.