Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico.
Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico.
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DOI：
10.1186/s12944-021-01526-5
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发表时间：
2021-10-10
影响因子：
4.5
通讯作者：
Bensinger SJ
中科院分区：
文献类型：
--
作者：
Flores YN;Amoon AT;Su B;Velazquez-Cruz R;Ramírez-Palacios P;Salmerón J;Rivera-Paredez B;Sinsheimer JS;Lusis AJ;Huertas-Vazquez A;Saab S;Glenn BA;May FP;Williams KJ;Bastani R;Bensinger SJ
非酒精性脂肪性肝病（NAFLD）是慢性肝病和肝硬化的主要原因。NAFLD是由脂质代谢改变介导的，已知的危险因素包括肥胖、代谢综合征和糖尿病。本研究的目的是通过对来自墨西哥成人队列研究的血清生物标本进行直接输注脂质组学，更好地了解NAFLD患者与对照组相比脂质组成的差异。在墨西哥进行的一项正在进行的纵向研究中，对98例NAFLD病例和100名健康对照者进行了巢式病例对照研究。排除酒精滥用后，通过升高的肝酶试验和肝脏超声或肝脏超声弹性成像确诊NAFLD病例，并确定100例对照组在6个月内至少连续两次丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST） （< 40 U/L）结果正常，2018年1月肝脏超声弹性成像结果正常。样品在Sciex脂质分析仪平台上分析，并以归一化血清体积定量。使用Lipidyzer靶向多反应监测表可以识别多达1100种脂质。采用协方差分析、随机森林分析和生成受试者操作特征（ROC）曲线来研究血脂与NAFLD之间的关系。NAFLD患者在血清胆固醇酯、溶血磷脂酰胆碱、鞘磷脂和甘油三酯（TAGs）的总量上存在差异，然而，其他脂质亚类与对照组相似。对单个TAG种类的分析显示，NAFLD患者血清中饱和脂肪酸酰尾的掺入增加。在调整了年龄、性别、体重指数和PNPLA3基因型后，10种脂质组合面板比ROC曲线下面积0.83更好地预测病例或对照状态。这些初步结果表明，与健康对照相比，NAFLD患者的血清脂质组存在差异，并提示评估TAGs的去饱和状态或特定的脂质面板可能是诊断NAFLD的有用临床工具。在线版本包含补充材料，可在10.1186/s12944-021-01526-5获得。
Nonalcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver disease and cirrhosis. NAFLD is mediated by changes in lipid metabolism and known risk factors include obesity, metabolic syndrome, and diabetes. The aim of this study was to better understand differences in the lipid composition of individuals with NAFLD compared to controls, by performing direct infusion lipidomics on serum biospecimens from a cohort study of adults in Mexico. A nested case-control study was conducted with a sample of 98 NAFLD cases and 100 healthy controls who are participating in an on-going, longitudinal study in Mexico. NAFLD cases were clinically confirmed using elevated liver enzyme tests and liver ultrasound or liver ultrasound elastography, after excluding alcohol abuse, and 100 controls were identified as having at least two consecutive normal alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (< 40 U/L) results in a 6-month period, and a normal liver ultrasound elastography result in January 2018. Samples were analyzed on the Sciex Lipidyzer Platform and quantified with normalization to serum volume. As many as 1100 lipid species can be identified using the Lipidyzer targeted multiple-reaction monitoring list. The association between serum lipids and NAFLD was investigated using analysis of covariance, random forest analysis, and by generating receiver operator characteristic (ROC) curves. NAFLD cases had differences in total amounts of serum cholesterol esters, lysophosphatidylcholines, sphingomyelins, and triacylglycerols (TAGs), however, other lipid subclasses were similar to controls. Analysis of individual TAG species revealed increased incorporation of saturated fatty acyl tails in serum of NAFLD cases. After adjusting for age, sex, body mass index, and PNPLA3 genotype, a combined panel of ten lipids predicted case or control status better than an area under the ROC curve of 0.83. These preliminary results indicate that the serum lipidome differs in patients with NAFLD, compared to healthy controls, and suggest that assessing the desaturation state of TAGs or a specific lipid panel may be useful clinical tools for the diagnosis of NAFLD. The online version contains supplementary material available at 10.1186/s12944-021-01526-5.
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影响因子：
4.3
作者：
Fleischman, Michael Wayne;Budoff, Matthew;Foster, Temitope
通讯作者：
Foster, Temitope
影响因子：
13.5
作者：
Browning, JD;Szczepaniak, LS;Hobbs, HH
通讯作者：
Hobbs, HH
影响因子：
3.8
作者：
Amarapurkar, Deepak;Kamani, Prafull;Deshpande, Anjali
通讯作者：
Deshpande, Anjali
DOI：
10.1097/meg.0b013e32832fcdf0
发表时间：
2010-01-01
影响因子：
2.1
作者：
Caballeria, Llorenc;Pera, Guillem;Maite Aizpurua, Miren
通讯作者：
Maite Aizpurua, Miren
影响因子：
4.4
作者：
Barr J;Caballería J;Martínez-Arranz I;Domínguez-Díez A;Alonso C;Muntané J;Pérez-Cormenzana M;García-Monzón C;Mayo R;Martín-Duce A;Romero-Gómez M;Lo Iacono O;Tordjman J;Andrade RJ;Pérez-Carreras M;Le Marchand-Brustel Y;Tran A;Fernández-Escalante C;Arévalo E;García-Unzueta M;Clement K;Crespo J;Gual P;Gómez-Fleitas M;Martínez-Chantar ML;Castro A;Lu SC;Vázquez-Chantada M;Mato JM
通讯作者：
Mato JM