Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
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裸鼠注射表皮干细胞和真皮乳头细胞促进毛囊再生的初步研究
DOI：
10.1007/s10561-020-09825-4
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发表时间：
2020-03
影响因子：
1.5
通讯作者：
Li Xinping
中科院分区：
文献类型：
--
作者：
Zhang Mingsheng;Ye Yan;Zhao Pin;Bai Liming;Li Xinping
器官再生疗法的最终目标是再生出功能完备的器官，以替代因疾病或损伤而受损的器官。尽管一些研究声称在某些动物模型中使用不同类型的细胞可促进……
The ultimate goal of organ regenerative therapy is to reproduce fully functional organs to replace which have been damaged as a result of diseases or injury. Although several studies claimed that using different types of cells in some animal models promote hair follicles regeneration, more researches can be done to develop a sufficient and efficient protocol to induce hair generation from different animal models. In this study, we investigated the therapeutic potentials for hair follicle formation by injecting a mixture of epidermal stem cells and dermal papilla cells. Those cells were isolated and culture-expanded. Then we randomly allocated 8 nude mice into two groups. The experiment group received an injection of a mixture that containing of epidermal stem cells and dermal papilla cells. The control group received injection of keratinocyte serum-free medium. The hair follicles regeneration was observed and the injection area was harvested for HE staining. 14 day later, the regenerated hair shafts were observed and HE staining indicated that the newly hair follicle formed the correct structures in experiment group. Furthermore, the mixture injection induced a regular and multilayered stratified epidermis and the epidermis contained of hair follicle-likes structures. Our data showed that injection of a mixture of epidermal stem cells and dermal papilla cells could induce hair follicles regeneration and well-ordered epidermis formation. This study emphasized that the rearrangement of the interactions during seed cells and the niches of the seed cells is essential and necessary for tissue-engineered construct success.
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影响因子：
16.6
作者：
通讯作者：
--
DOI：
10.1016/s0093-3619(08)70911-0
发表时间：
2008
期刊：
Yearbook of Dermatology and Dermatologic Surgery
影响因子：
--
作者：
B. Thiers
通讯作者：
B. Thiers
影响因子：
4.6
作者：
Asakawa, Kyosuke;Toyoshima, Koh-ei;Ishibashi, Naoko;Tobe, Hirofumi;Iwadate, Ayako;Kanayama, Tatsuya;Hasegawa, Tomoko;Nakao, Kazuhisa;Toki, Hiroshi;Noguchi, Shotaro;Ogawa, Miho;Sato, Akio;Tsuji, Takashi
通讯作者：
Tsuji, Takashi
影响因子：
2.7
作者：
Shao-Hai, Qi;Po, Liu;Tian-Zenq, Li
通讯作者：
Tian-Zenq, Li
影响因子：
6
作者：
Jose Leiros, Gustavo;Gabriela Kusinsky, Ana;Eugenia Balana, Maria
通讯作者：
Eugenia Balana, Maria