Estimating energy expenditure in mice using an energy balance technique.

Estimating energy expenditure in mice using an energy balance technique.
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DOI:
10.1038/ijo.2012.105
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发表时间:
2013-03
影响因子:
4.9
通讯作者:
Leibel, R. L.
Leibel, R. L.
中科院分区:
医学2区
文献类型:
--
作者:
Ravussin, Y.;Gutman, R.;LeDuc, C. A.;Leibel, R. L.

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比较小鼠中使用能量平衡技术(TEEbal:食物能量摄入和身体成分变化)与间接量热法 (TEEIC) 估计能量消耗的准确性。使用能量平衡(热量摄入减去身体能量储存的变化)方法估算 32 只雄性 C57BL/6J 小鼠 37 天的能量消耗。还通过间接量热法测量了相同动物的能量消耗。对这些措施进行了比较。两种方法高度相关(r2 = 0.87:TEEbal = 1.07 * TEEIC − 0.22,p < 0.0001)。通过 Bland-Altman 分析,TEEbal 估计值略高于 TEEIC 估计值(偏差 = 0.55 kcal/24h)(4.6±1.5%;p < 0.05)。 TEEbal 可以在“家庭笼子”中进行,提供准确的综合长期能量消耗测量,同时最大限度地减少使用间接量热系统可能带来的潜在混杂压力。该技术还可用于评估长期能量摄入量。
To compare, in mice, the accuracy of estimates of energy expenditure using an energy balance technique (TEEbal : food energy intake and body composition change) versus indirect calorimetry (TEEIC). In 32 male C57BL/6J mice energy expenditure was estimated using an energy balance (caloric intake minus change in body energy stores) method over a 37 day period. Energy expenditure was also measured in the same animals by indirect calorimetry. These measures were compared. The two methods were highly correlated (r2 = 0.87: TEEbal = 1.07 * TEEIC − 0.22, p < 0.0001). By Bland-Altman analysis, TEEbal estimates were slightly higher (4.6±1.5%; p < 0.05) than TEEIC estimates (Bias = 0.55 kcal/24h). TEEbal can be performed in “home cages” and provides an accurate integrated long-term measurement of energy expenditure while minimizing potentially confounding stress that may accompany the use of indirect calorimetry systems. The technique can also be used to assess long term energy intake.
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