Precise quantification of nanoparticle internalization.
Precise quantification of nanoparticle internalization.
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DOI：
10.1021/nn400243d
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发表时间：
2013-06-25
期刊：
影响因子：
17.1
通讯作者：
Zasadzinski, Joseph Anthony
中科院分区：
文献类型：
--
作者：
Gottstein, Claudia;Wu, Guohui;Wong, Benjamin J.;Zasadzinski, Joseph Anthony
关键词：
纳米颗粒为人类疾病的诊断和治疗应用开辟了令人兴奋的新途径，并且靶向纳米颗粒越来越多地用作特定的药物输送载体。纳米颗粒内化的精确量化对于测量物理和化学性质对纳米颗粒摄取到靶细胞或负责快速清除的细胞的影响非常重要。纳米粒子的内化已通过各种技术进行测量，但由于缺乏普遍接受的标准化测定，不同实验室之间的数据可比性受到阻碍。此外，关联粒子和内化粒子之间的区别多年来一直是一个挑战，尽管这种区别对于大多数研究问题至关重要。以前使用的验证细胞内位置的方法通常不是定量的，并且不适合高通量分析。在这里，我们开发了一个数学模型，它将高通量流式细胞术测量的数据与定量共聚焦显微镜的数据相结合。这里描述的通用方法将成为生物医学纳米技术研究中的有用工具。然后应用该方法来测量囊泡表面涂层对其被网状内皮系统（RES）细胞内化的影响。 RES细胞负责纳米颗粒的快速清除，由此产生的快速血液清除是纳米颗粒生物医学应用的主要挑战之一。用长链聚乙二醇涂覆的囊泡显示出 RES 细胞内化程度较低的趋势。
Nanoparticles have opened new exciting avenues for both diagnostic and therapeutic applications in human disease, and targeted nanoparticles are increasingly used as specific drug delivery vehicles. The precise quantification of nanoparticle internalization is of importance to measure the impact of physical and chemical properties on the uptake of nanoparticles into target cells or into cells responsible for rapid clearance. Internalization of nanoparticles has been measured by various techniques, but comparability of data between different labs is impeded by lack of a generally accepted standardized assay. Furthermore, the distinction between associated and internalized particles has been a challenge for many years, although this distinction is critical for most research questions. Previously used methods to verify intracellular location are typically not quantitative and do not lend themselves to high throughput analysis. Here we developed a mathematical model which integrates the data from high throughput flow cytometry measurements with data from quantitative confocal microscopy. The generic method described here will be a useful tool in biomedical nanotechnology studies. The method was then applied to measure the impact of surface coatings of vesosomes on their internalization by cells of the reticuloendothelial system (RES). RES cells are responsible for rapid clearance of nanoparticles, and the resulting fast blood clearance is one of the major challenges in biomedical applications of nanoparticles. Coating of vesosomes with long chain polyethylene glycol showed a trend for lower internalization by RES cells.
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影响因子：
3.4
作者：
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通讯作者：
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