Central administration of corticotrophin releasing hormone but not arginine vasopressin stimulates the secretion of luteinizing hormone in rams in the presence and absence of testosterone.
Central administration of corticotrophin releasing hormone but not arginine vasopressin stimulates the secretion of luteinizing hormone in rams in the presence and absence of testosterone.
复制标题
在存在或不存在睾酮的情况下，中央施用促肾上腺皮质激素释放激素而不是精氨酸加压素会刺激公羊黄体生成素的分泌。
作者：
AJ Tilbrook;B. Canny;BJ Stewart;MD Serapiglia;IJ Clarke
本研究测试的假设，中央管理的促肾上腺皮质激素释放激素（CRH）和/或精氨酸加压素（AVP）将影响LH的分泌公羊和睾酮是必要的，这些行动的发生。在将100微升载体/模拟脑脊液（CSF）或含有25微克CRH、25微克AVP或25微克每种肽的模拟CSF通过引导插管输注到第三脑室中1小时期间，在去势公羊中测量血浆LH水平。这些脑室内（i. c. v.）在注射（i.m.）每12小时油或8毫克丙酸睾酮7天。在i. c. v.输注前4小时、输注期间和输注后4小时，每10分钟采集一次血样。溶媒输注不影响任何内分泌参数。与此相反，LH的血浆浓度和LH脉冲的幅度显着增加期间和之后输注CRH，这种效果是不受影响的去势公羊是否与丙酸睾酮或是否CRH与AVP联合给药。单独输注AVP不影响LH分泌。LH脉冲频率和FSH血浆浓度未随任何i. c. v.治疗而变化。CRH和AVP输注可显著增加血浆皮质醇浓度。CRH和AVP联合静脉输注期间和之后达到的皮质醇血浆浓度大于单独用AVP治疗的结果，但与CRH相似。丙酸睾酮对皮质醇水平没有影响。这些结果表明，CRH而不是AVP能够在中枢或垂体水平上作用于公羊以增加LH的分泌，并且这些作用不受睾酮的影响。CRH对LH分泌的刺激作用是增加GnRH脉冲的幅度和/或垂体对GnRH作用的反应性，而对GnRH脉冲的频率没有影响。FSH的分泌不受CRH或AVP的影响。CRH增加LH脉冲幅度的作用发生在皮质醇水平增加的情况下，进一步加强了我们的信念，即这种肾上腺类固醇不会影响该物种的生殖轴。
This study tested the hypothesis that central administration of corticotrophin-releasing hormone (CRH) and/or arginine vasopressin (AVP) will affect the secretion of LH in rams and that testosterone is necessary for these actions to occur. Plasma LH levels were measured in castrated rams during 1 h infusion of either 100 microliter vehicle/mock cerebrospinal fluid (CSF) or mock CSF containing 25 microgram CRH, 25 microgram AVP or 25 microgram of each peptide through guide cannulae into the third cerebral ventricle. These intracerebroventricular (i.c.v.) infusions were given to the castrated rams following injections (i.m.) each 12 h of oil or 8 mg testosterone propionate for 7 days. Blood samples were collected every 10 min for 4 h before i.c.v. infusion, during infusion and for 4 h following the infusion. Infusion of vehicle did not affect any endocrine parameters. In contrast, the plasma concentrations of LH and the amplitude of LH pulses were increased significantly during and following infusion of CRH, and this effect was not influenced by whether the castrated rams were treated with testosterone propionate or whether the CRH was administered in combination with AVP. Infusion of AVP alone did not affect LH secretion. The frequency of LH pulses and the plasma concentrations of FSH did not change with any of the i.c.v. treatments. The plasma concentrations of cortisol were significantly increased by CRH and AVP infusions. The plasma concentrations of cortisol achieved during and following i.c.v. infusion of CRH and AVP combined were greater than the concentrations achieved as a result of treatment with AVP alone but were similar to those with CRH. There was no effect of testosterone propionate on cortisol levels. These results show that CRH, but not AVP, is capable of acting either centrally or at the pituitary level to increase the secretion of LH in rams and these actions are not affected by testosterone. The stimulatory effects of CRH on LH secretion are to increase the amplitude of GnRH pulses and/or the responsiveness of the pituitary to the actions of GnRH with no effect on the frequency of GnRH pulses. The secretion of FSH in rams is not influenced by either CRH or AVP. The effect of CRH to increase LH pulse amplitude occurs in the face of increased cortisol levels, further reinforcing our belief that this adrenal steroid does not affect the reproductive axis in this species.
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DOI：
10.1210/edrv-16-2-177
发表时间：
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Endocrine reviews.
影响因子：
--
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DOI：
10.1210/jcem-65-2-262
发表时间：
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期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
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通讯作者：
Ferin,M
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影响因子：
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