Microstructural analysis of secondary pulmonary lobule imaged by synchrotron radiation micro CT using offset scan mode
Microstructural analysis of secondary pulmonary lobule imaged by synchrotron radiation micro CT using offset scan mode
复制标题
采用偏置扫描模式的同步辐射显微CT成像次级肺小叶的显微结构分析
DOI：
10.1117/12.845583
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发表时间：
2010
影响因子：
--
通讯作者：
Harumi Itoh
中科院分区：
文献类型：
--
作者：
Y. Kawata;K. Kageyama;Noboru Niki;Keiji Umetani;K. Yada;H. Ohamatsu;Noriyuki Moriyama;Harumi Itoh
在临床常规的CT检查中，与小叶解剖相关的异常识别在肺异常的诊断和鉴别诊断中变得越来越重要。本研究的目的是利用几微米范围内的各向同性空间分辨率分析小叶解剖结构的微观结构，以定量描述结构与异常之间的关系。最近的商用微型CT扫描仪在肺微结构成像中起着至关重要的作用。然而，由于难以在扫描视野之外成像肺次级小叶和肺实质造影剂有限，因此仅进行了有限数量的尝试。本文论证了同步辐射微CT （SRμCT）采用偏移扫描方式对肺次级小叶进行显微结构分析的能力。利用同步辐射装置（SPring-8）的SRμCT （15 keV）偏移扫描模式，对充气固定后的肺标本进行扫描，扫描分辨率为5.87x5.87x5.87 μm3。三维SRμCT图像堆叠2624片（每片7287x7287体素），覆盖肺标本中包含的肺次级小叶。适当分割的适当阈值被交互式地确定为代表次级肺小叶的兴趣体积。在将分割的二值图像转换为骨架化的表面表示后，每个体素被分类为曲线、表面或连接点。利用提供几何信息的体素分类结果对小叶间隔区域进行交互提取。考虑到相同的衰减值和连通性，采用种子技术提取小叶气道、动脉和静脉的每个组成部分。利用偏移扫描模式从SRμCT获得的体积图像使小叶解剖的三维显微结构分析成为可能。
The recognition of abnormalities relative to the lobular anatomy has become increasingly important in the diagnosis and differential diagnosis of lung abnormalities at clinical routines of CT examinations. The purpose of this study is to analyze microstructure of the lobular anatomy with isotropic spatial resolution in the range of several micrometers to quantitatively describe relation between the architectures and abnormalities. Recent commercial micro CT scanners play a vital role in imaging the lung micro-architectures. However, only a limited number of attempts have been conducted because of difficulties to image the secondary pulmonary lobule beyond the scan field of view and the limited contrast lung parenchyma. This paper demonstrates the ability of synchrotron radiation micro CT (SRμCT) using offset scan mode in microstructural analysis of the secondary pulmonary lobule. The inflated and fixed lung specimen was imaged with resolution of 5.87x5.87x5.87 μm3 by using offset scan mode of the SRμCT (15 keV) at the synchrotron radiation facility (SPring-8). The 3-D SRμCT image which was stacked 2624 slices (each slice:7287x7287 voxels) covered the secondary pulmonary lobule being included in the lung specimen. A proper threshold value for appropriate segmentation was interactively determined to the volume of interest representing the secondary pulmonary lobule. Following transformation of the segmented binary image to a skeletonized surface representation, each voxel was classified as a curve, surface, or junction. The interlobular septa region was extracted interactively by using the voxel classification result which offered geometrical information. Each component of lobular airway, artery, and vein were extracted by using a seeding technique, considering equal attenuation values and connectivity. The resulting volumetric image from the SRμCT using offset scan mode made 3-D microstructural analysis of the lobular anatomy possible.
影响因子：
3.3
作者：
Parameswaran, Harikrishnan;Bartolak-Suki, Erzsebet;Suki, Bela
通讯作者：
Suki, Bela
DOI：
10.1513/pats.200508-080ds
发表时间：
2005-01-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Ritman, Erik L
通讯作者：
Ritman, Erik L
影响因子：
3.3
作者：
Tsuda, A.;Filipovic, N.;Schittny, J. C.
通讯作者：
Schittny, J. C.