100 Hz Electroacupuncture Alleviated Chronic Itch and GRPR Expression Through Activation of Kappa Opioid Receptors in Spinal Dorsal Horn.
100 Hz Electroacupuncture Alleviated Chronic Itch and GRPR Expression Through Activation of Kappa Opioid Receptors in Spinal Dorsal Horn.
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100 Hz 电针通过激活脊髓背角 Kappa 阿片受体缓解慢性瘙痒和 GRPR 表达
DOI：
10.3389/fnins.2021.625471
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发表时间：
2021
影响因子：
4.3
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Li HP;Wang XY;Chen C;Li JJ;Yu C;Lin LX;Yu ZE;Jin ZY;Zhu H;Xiang HC;Hu XF;Cao J;Jing XH;Li M
背景：临床研究表明，电针可以缓解慢性瘙痒。脊髓背角的胃泌素释放肽受体(GRPR)和强啡肽(Dyn)分别对瘙痒有正向或负向调节作用。然而，电针哪个频率能有效地缓解慢性瘙痒和减少GRPR的表达，脊髓中的Dyn-A是否参与了电针止痒作用的潜在机制尚不清楚。方法采用丙酮-乙醚(1：1)混合液(简称AEW)诱导慢性瘙痒皮肤干燥模型。电针治疗曲池穴和合谷穴。Western印迹法检测GRPR和dyn-A的表达。免疫荧光法检测Dyn-A的表达。结果AEW可引起明显的自发划痕，增强GRPR的表达，降低dyn-A的表达。与假电针组、2赫兹电针组和15赫兹电针组相比，100赫兹电针能最有效地缓解慢性瘙痒，减少GRPR的表达，增加Dyn-A在颈背角的表达。此外，腹腔注射kappa阿片受体(KORS)拮抗剂Nor-Binaltorphimine二盐酸盐(Nor-BNI)可显著逆转100 Hz电针对瘙痒行为和GRPR表达的抑制作用。结论100 Hz电针是通过激活脊髓背角Kors抑制慢性瘙痒和GRPR表达的最有效频率，可有效指导临床治疗，提高针刺止痒效果。
Background Clinical studies have shown that electroacupuncture (EA) alleviates chronic itch. Gastrin-releasing peptide receptor (GRPR) and dynorphin (DYN) in the spinal dorsal horn positively or negatively regulate itch, respectively. However, which frequency of EA is effective on relieving chronic itch and reducing the expression of GRPR, whether DYN-A in the spinal cord is involved in the underlying mechanism of the antipruritus effect of EA remains unknown. Methods The mixture of acetone and diethyl ether (1:1) [designated as AEW (acetone/diethyl ether and water) treatment] was used to induce the dry skin model of chronic itch. EA was applied to Quchi (LI11) and Hegu (LI4). Western blot was used to detect the expression of GRPR and DYN-A. Immunofluorescence was used to detect the expression of DYN-A. Results The AEW administration induced remarkable spontaneous scratching, enhanced the expression of GRPR, and reduced the expression of DYN-A. Compared with the sham EA, 2 Hz EA, or 15 Hz EA group, 100 Hz EA was the most effective frequency for relieving chronic itch, reducing the expression of GRPR, and increasing the expression of DYN-A in the cervical dorsal horn. Furthermore, intraperitoneal injection of kappa opioid receptors (KORs) antagonist nor-Binaltorphimine dihydrochloride (nor-BNI) significantly reversed the effect of 100 Hz EA on the inhibition of both itching behavior and GRPR expression. Conclusion EA at 100 Hz is the most effective frequency that inhibits chronic itch and GRPR expression through activation of KORs in the spinal dorsal horn, which can effectively guide the clinical treatment and improve the antipruritic effect of acupuncture.
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DOI：
10.1155/2013/319124
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Chen YH;Yang HY;Lin CH;Dun NJ;Lin JG
通讯作者：
Lin JG
影响因子：
0.3
作者：
Han, Jae-Bok;Kim, Chan Woo;Min, Byung-Il
通讯作者：
Min, Byung-Il
电针通过降低饮食诱导肥胖大鼠神经蛋白 Y/Agouti 相关蛋白水平并抑制蛋白酪氨酸磷酸酶 1B 表达改善胰岛素抵抗
影响因子：
1
作者：
Liu, Xia;He, Jun-Feng;Jiang, Peng-Fei
通讯作者：
Jiang, Peng-Fei
DOI：
10.1007/978-3-662-44605-8_16
发表时间：
2015-01-01
期刊：
PHARMACOLOGY OF ITCH
影响因子：
--
作者：
Cowan, Alan;Kehner, George B.;Inan, Saadet
通讯作者：
Inan, Saadet
影响因子：
16.2
作者：
Kardon AP;Polgár E;Hachisuka J;Snyder LM;Cameron D;Savage S;Cai X;Karnup S;Fan CR;Hemenway GM;Bernard CS;Schwartz ES;Nagase H;Schwarzer C;Watanabe M;Furuta T;Kaneko T;Koerber HR;Todd AJ;Ross SE
通讯作者：
Ross SE