Electroacupuncture Attenuates 5'-Guanidinonaltrindole-Evoked Scratching and Spinal c-Fos Expression in the Mouse.
Electroacupuncture Attenuates 5'-Guanidinonaltrindole-Evoked Scratching and Spinal c-Fos Expression in the Mouse.
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DOI：
10.1155/2013/319124
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Lin JG
中科院分区：
文献类型：
--
作者：
Chen YH;Yang HY;Lin CH;Dun NJ;Lin JG
本研究旨在观察电针对小鼠强迫性抓挠行为及皮下注射（s.c.）将已知的致清剂5′-胍基那曲吲哚（GNTI）施用于颈部。电针合谷（LI 4）和曲池（LI 11）穴位在2 Hz，但不是100 Hz，减弱GNTI诱发的抓挠。在用μ阿片受体拮抗剂纳洛酮预处理的小鼠中，2 Hz的EA没有减弱GNTI诱发的抓挠，而在用κ阿片受体拮抗剂nor-binaltorphimine预处理的小鼠中，2 Hz的EA确实减弱了GNTI诱发的抓挠。此外，皮内（i.d.）给予选择性μ阿片受体激动剂[d-Ala 2，N-Me-Phe 4，Gly 5-ol]-脑啡肽乙酸酯（DAMGO）减弱GNTI诱发的抓挠行为，而s.c. DAMGO的给药无效。GNTI引起c-Fos在颈脊髓背角浅层外侧的表达。电针LI 4和LI 11可减少GNTI诱导的c-Fos阳性核数。可以得出结论，2 Hz电针对LI 4和LI 11的应用减弱了GNTI诱导的小鼠抓挠行为，外周μ阿片系统至少部分参与了电针的抗瘙痒作用。
The present study was undertaken to investigate the influence of electroacupuncture (EA) on compulsive scratching in mice and c-Fos expression elicited by subcutaneous (s.c.) administration of a known puritogen, 5′-guanidinonaltrindole (GNTI) to the neck. Application of EA to Hegu (LI4) and Quchi (LI11) acupoints at 2 Hz, but not 100 Hz, attenuated GNTI-evoked scratching. In mice pretreated with the µ opioid receptor antagonist naloxone, EA 2 Hz did not attenuate GNTI-evoked scratching, whereas EA at 2 Hz did attenuate GNTI-evoked scratching in mice pretreated with the κ opioid receptor antagonist nor-binaltorphimine. Moreover, intradermal (i.d.) administration of the selective µ opioid receptor agonist [d-Ala2, N-Me-Phe4, Gly5-ol]-enkephalin acetate (DAMGO) attenuated GNTI-evoked scratching behavior, while s.c. administration of DAMGO was ineffective. GNTI provoked c-Fos expression on the lateral side of the superficial layer of the dorsal horn of the cervical spinal cord. Application of 2 Hz EA to LI4 and LI11 decreased the number of c-Fos positive nuclei induced by GNTI. It may be concluded that application of 2 Hz EA to LI4 and LI11 attenuates scratching behavior induced by GNTI in mice and that the peripheral µ opioid system is involved, at least in part, in the anti-pruritic effects of EA.
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