Spatial organization of the flow of genetic information in bacteria.
Spatial organization of the flow of genetic information in bacteria.
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作者：
真核细胞在空间上组织mRNA的过程，如翻译和mRNA衰变。在细菌细胞中，成熟mRNA的空间分布仍然不明确，这一点就不清楚了。使用一种敏感的、基于荧光原位杂交的方法，我们在这里发现，在新月茎杆菌和大肠杆菌中，染色体表达的mrna在其一生中基本上从转录位点显示有限的分散。我们估计表观扩散系数至少比预期的自由扩散的mRNA低2个数量级，并提供证据表明这种mRNA定位限制了核糖体的流动性。此外，月牙菇RNase E似乎与DNA相关，独立于其mRNA底物。总的来说，我们的研究结果表明，细菌可以利用染色体布局作为模板，在空间上组织翻译和潜在的mRNA衰变。这种以染色体为中心的组织对细胞生理学和我们对细菌基因表达的理解具有重要意义。
Eukaryotic cells spatially organize mRNA processes such as translation and mRNA decay. Much less is clear in bacterial cells where the spatial distribution of mature mRNA remains ambiguous. Using a sensitive, quantitative fluorescence in situ hybridization based-method, we show here that in Caulobacter crescentus and Escherichia coli, chromosomally-expressed mRNAs largely display limited dispersion from their site of transcription during their lifetime. We estimate apparent diffusion coefficients at least 2 orders of magnitude lower than expected for freely diffusing mRNA, and provide evidence in C. crescentus that this mRNA localization restricts ribosomal mobility. Furthermore, C. crescentus RNase E appears associated with the DNA independently of its mRNA substrates. Collectively, our findings reveal that bacteria can spatially organize translation and potentially mRNA decay by using the chromosome layout as a template. This chromosome-centric organization has important implications for cellular physiology and for our understanding of gene expression in bacteria.
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影响因子：
14.9
作者：
Guet, Calin C.;Bruneaux, Luke;Min, Taejin L.;Siegal-Gaskins, Dan;Figueroa, Israel;Emonet, Thierry;Cluzel, Philippe
通讯作者：
Cluzel, Philippe
影响因子：
56.9
作者：
Maamar, Hedia;Raj, Arjun;Dubnau, David
通讯作者：
Dubnau, David
影响因子：
3.6
作者：
Avedissian, M;Gomes, SL
通讯作者：
Gomes, SL
影响因子：
64.5
作者：
Domian, IJ;Quon, KC;Shapiro, L
通讯作者：
Shapiro, L
DOI：
10.1073/pnas.0404443101
发表时间：
2004-08-03
影响因子：
11.1
作者：
Golding, I;Cox, EC
通讯作者：
Cox, EC