Differential expression of laminin isoforms and beta 4 integrin epitopes in the basement membrane zone of normal human skin and basal cell carcinomas.
Differential expression of laminin isoforms and beta 4 integrin epitopes in the basement membrane zone of normal human skin and basal cell carcinomas.
复制标题
正常人皮肤和基底细胞癌基底膜区层粘连蛋白亚型和β4整合素表位的差异表达。
DOI：
10.1111/1523-1747.ep12457080
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Uitto,J
中科院分区：
文献类型：
--
作者：
Sollberg,S;Peltonen,J;Uitto,J
用识别3种不同层粘蛋白亚型的5种亚基多肽和β-4整合素表位的单抗，间接免疫荧光法研究了正常人皮肤和基底细胞癌的基底膜带生物学。层粘连蛋白抗体可针对经典层粘连蛋白的A、B1、B2链，针对Merosin的M链，或针对S-层粘连蛋白的S链。正常人皮肤的免疫染色显示A、B1和B2链表位的抗体有很强的信号。用抗M链抗体检测到微弱的免疫信号，而即使用透明质酸酶预处理切片后，也检测不到S链表位。因此，正常人皮肤表皮-真皮交界处的层粘连蛋白主要是经典类型，存在一些Merosin分子。6例结节性基底细胞癌的染色显示A、B1和B2链表位呈线性分布，但与正常皮肤相比，识别M链表位的抗体产生了强烈的免疫信号，S链表位也很容易被检测到。层粘连蛋白的潜在受体β-4整合素的染色显示，在正常皮肤和基底细胞癌的浅表部位都有强烈的染色反应。然而，病变深层的免疫荧光模式是分散和中断的。因此，结节性基底细胞癌基底膜相对于层粘连蛋白亚型的组成改变，以及它们与可能的细胞表面受体β4整合素的相互作用，可能改变肿瘤岛的遏制，促进基底细胞癌的局部侵袭行为。
The basement membrane zone biology of normal human skin and basal cell carcinomas was explored by indirect immunofluorescence with monoclonal antibodies recognizing five subunit polypeptides of three different laminin isoforms as well as the β4 integrin epitopes. The laminin antibodies were specific for A, B1, and B2 chains of classic laminin, for the M chain of merosin, or for the S chain in S-laminin. Immunostaining of normal human skin revealed a strong signal with antibodies for A, B1, and B2 chain epitopes. A weak immunosignal was detected with an anti — M chain antibody, whereas the S-chain epitopes were undetectable, even following pretreatment of sections with hyaluronidase. Thus, the laminin at the epidermal-dermal junction of normal human skin is primarily of the classic type, with some merosin molecules being present. The staining of six nodular basal cell carcinomas revealed the presence of A, B1, and B2 chain epitopes in a linear pattern, but, in contrast to normal skin, the antibody recognizing M-chain epitopes yielded a strong immunosignal, and S-chain epitopes could also be readily detected. Staining for β4 integrins, potential receptors for laminin, revealed a strong staining reaction in normal skin as well as in the superficial portions of the basal cell carcinoma. However, the immunofluorescence pattern in the deeper portions of the lesions was scattered and interrupted. Thus, altered composition of the basement membrane of nodular basal cell carcinomas with respect to laminin isoforms and their interactions with putative cell-surface receptors, the β4 integrins, may change the containment of the tumor islands, contributing to the local aggressive behavior of basal cell carcinomas.
登录
查看更多内容
基底细胞上皮瘤中的纤连蛋白：来源和意义。
DOI：
10.1111/1523-1747.ep12259701
发表时间：
1984
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Grimwood,RE;Huff,JC;Harbell,JW;Clark,RA
通讯作者：
Clark,RA
DOI：
10.1091/mbc.1.10.731
发表时间：
1990-09-01
期刊：
CELL REGULATION
影响因子：
--
作者：
ENGVALL, E;EARWICKER, D;SANES, JR
通讯作者：
SANES, JR
人类皮肤疾病中的胶原酶：成纤维细胞培养物中表达的保真度。
DOI：
--
发表时间：
1983
期刊：
Monographs in pathology
影响因子：
--
作者：
Bauer,EA;Uitto,J;Eisen,AZ
通讯作者：
Eisen,AZ
基底细胞上皮瘤中纤连蛋白和层粘连蛋白的分布。
DOI：
10.1111/1523-1747.ep12555545
发表时间：
1983
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
D. Nelson;C. Little;G. Balian
通讯作者：
G. Balian
人类皮肤中 β 4 整合素的表达：表皮分布与 β 1 整合素表位的比较，以及培养的角质形成细胞中钙和维生素 D3 的调节。
DOI：
10.1111/1523-1747.ep12481896
发表时间：
1991
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Ryynänen,J;Jaakkola,S;Engvall,E;Peltonen,J;Uitto,J
通讯作者：
Uitto,J