Re-classification within the serogroups O3 and O8 of Citrobacter strains.
Re-classification within the serogroups O3 and O8 of Citrobacter strains.
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DOI：
10.1186/s12866-017-1078-3
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发表时间：
2017-07-27
期刊：
影响因子：
4.2
通讯作者：
Gamian A
中科院分区：
文献类型：
--
作者：
Katzenellenbogen E;Staniszewska M;Kocharova NA;Mieszała M;Korzeniowska-Kowal A;Górska S;Knirel YA;Gamian A
柠檬酸菌株是机会性病原体，通常负责严重的肠以及肠外疾病，因此应设置仍用于诊断诊断的O抗原方案以进行适当的血清分型。到目前为止，已经阐明了30多种不同的柠檬酸O-抗原（脂多糖脂多糖链的O-可糖链）的结构。但是，免疫化学研究仍然需要阐明几个异质血清群中菌株之间的关系。其中包括复杂的血清群O3和O8，分别由20和7菌株代表，这是当前工作的主题。早些时候，已经确定了针对柠檬酸菌菌菌株BE35/57（PCM 1508）和柠檬杆菌O8菌株BE64/57（PCM 1536）的O-杆菌结构。
从不同菌株中归因于血清群O3和O8的柠檬酸脂蛋白糖酸进行的血清学研究（免疫印迹）表明，这些血清群应分为非交叉反应的亚组。根据化学分析以及1H和13C NMR光谱的结果，已经建立了来自PCM 1504（O6）和PCM 1573（O2）的柠檬酸杆菌O-抗原的结构。化学数据与血清学分析相结合表明，应将几种柠檬酸菌株重新分类为其他血清群。
本文描述的对柠檬菌LP进行的免疫化学研究表明，重新分类的权宜之计：1）PCM 1504和PCM 1573菌株从血清群O6和O2到血清组O3和O8的PCM 1573，分别为2），2）菌株PCM 1503和PCM 1503和PCM 1505 serogroups O3和O8和O8和O8和O8和O8和O8 o3 and o3 anga and o3 anga and o3 ana secy o3 anga secy o3 ana seply o3 anga secy o3 ana secy o3 anga secy o3 anga secy o3 ana secy o3 anga secy o3 anga。
Citrobacter strains are opportunistic pathogens often responsible for serious enteric as well as extra-intestinal diseases, and therefore the O-antigenic scheme, still in use in diagnostic identification, should be set for proper serotyping. The structures of more than 30 different Citrobacter O-antigens (O-polysaccharide chains of the lipopolysaccharides) of 43 Citrobacter O-serogroups have been elucidated so far. However, relationships between strains in several heterogeneous serogroups still need to be clarified by immunochemical studies. These include complex serogroups O3 and O8, represented by 20 and 7 strains, respectively, which are the subject of the present work. Earlier, the O-polysaccharide structures have been determined for Citrobacter O3 strain Be35/57 (PCM 1508) and Citrobacter O8 strain Be64/57 (PCM 1536). Serological studies (immunoblotting) carried out on Citrobacter lipopolysaccharides from different strains ascribed to serogroups O3 and O8 showed that each of these serogroups should be divided into non-cross-reacting subgroups. Based on the results of chemical analyses and 1H and 13C NMR spectroscopy the structure of Citrobacter O-antigens from strains PCM 1504 (O6) and PCM 1573 (O2) have been established. Chemical data combined with serological analyses showed that several Citrobacter strains should be reclassified into other serogroups. Immunochemical studies carried out on Citrobacter LPS, described in this paper, showed the expediency of reclassification of: 1) strains PCM 1504 and PCM 1573 from serogroups O6 and O2 to serogroups O3 and O8, respectively, 2) strains PCM 1503 and PCM 1505 from serogroups O3 and O8 to new serogroups O3a and O8a, respectively.
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影响因子：
2.9
作者：
TSAI, CM;FRASCH, CE
通讯作者：
FRASCH, CE
DOI：
10.1016/s0172-5599(80)80053-8
发表时间：
1980-01-01
期刊：
ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY
影响因子：
--
作者：
AHAMED, NM;RADZIEJEWSKALEBRECHT, J;MAYER, H
通讯作者：
MAYER, H
DOI：
10.1099/00207713-43-4-777
发表时间：
1993-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
BRENNER, DJ;OCONNOR, SP;STEIGERWALT, AG
通讯作者：
STEIGERWALT, AG
DOI：
10.1073/pnas.76.9.4350
发表时间：
1979-01-01
影响因子：
11.1
作者：
TOWBIN, H;STAEHELIN, T;GORDON, J
通讯作者：
GORDON, J
DOI：
10.1046/j.1432-1033.2003.03640.x
发表时间：
2003-07-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Katzenellenbogen, E;Kocharova, NA;Knirel, YA
通讯作者：
Knirel, YA