Simultaneously qualitative and quantitative analysis of the representative components in Kadsura heteroclita stem by UHPLC-Q-Orbitrap HRMS.
Simultaneously qualitative and quantitative analysis of the representative components in Kadsura heteroclita stem by UHPLC-Q-Orbitrap HRMS.
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DOI：
10.38212/2224-6614.3348
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发表时间：
2021-06-15
影响因子：
3.6
通讯作者：
中科院分区：
文献类型：
--
作者：
红蓟是土家族药用植物，用于治疗风湿性关节炎。到目前为止，KHS中复杂的化学成分尚不清楚，KHS中主要化合物的水平也不清楚。本研究建立了一种新的超高效液相色谱-Q-Orbitrap高分辨质谱法，用于KHS的同时定性定量分析。共鉴定出204个化合物，包括三萜类、木脂素类、倍半萜类、脂肪酸类、酚酸类和黄酮类，其中100多个化合物为首次在KHS中发现。使用相同的方法，成功地定量了12种代表性的生物活性成分。通过线性、检测限、定量限、精密度、稳定性、回收率和基质效应对该方法进行了充分验证，并将其用于定量4批KHS中的12种代表性化合物。由于该方法能够进行回顾性数据分析，并且对单次运行中的分析物数量没有上限，因此将来可以应用于定量KHS的更多活性成分。
Kadsura heteroclita (Roxb) Craib stem (KHS) is a medicinal plant used for the treatment of rheumatism arthritis diseases in Tujia ethnomedicine. Thus far, the complex chemical compositions in KHS are not clear, and the levels of the major compounds in KHS are not well understood. In this study, a novel UHPLC-Q-Orbitrap HRMS method was established for the simultaneous quali-quantitative analysis of KHS. A total of 204 compounds were identified, including triterpenoids, lignans, sesquiterpenes, fatty acids, phenolic acids, and flavonoids, more than 100 of which were first discovered in KHS. Using the same method, 12 representative bioactive components were successfully quantified. The method was fully validated by linearity, LOD, LOQ, precision, stability, recovery, and matrix effects, and it was applied to quantify the 12 representative compounds in 4 batches of KHS. As this method enables retrospective data analysis and has no upper limit to the number of analytes in a single run, it can be applied to quantify more active components of KHS in the future.
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