Oo-site: A dashboard to visualize gene expression during Drosophila oogenesis suggests meiotic entry is regulated post-transcriptionally.
Oo-site: A dashboard to visualize gene expression during Drosophila oogenesis suggests meiotic entry is regulated post-transcriptionally.
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OO位点：一个可视化果蝇卵子发生过程中基因表达的仪表板表明减数分裂进入是转录后调控的。
作者：
确定如何控制干细胞分化对于理解退行性疾病的病因学和设计再生疗法具有重要意义。干细胞模型系统的体内分析揭示了干细胞自我更新和分化的调控模式。雌性果蝇生殖腺的生殖区，其中既有生殖系干细胞，也有体干细胞，就是这样一个模型系统。大量mRNA测序（RNA-seq）、单细胞RNA-seq（scRNA-seq）和mRNA的大量翻译效率（polysome-seq）可用于干细胞及其在果蝇生殖室内的分化后代。然而，这些数据的可视化是阻碍了缺乏一个工具来空间映射基因表达和翻译数据的生殖。在这里，我们开发了Oo-site（https：//www.ranganlab.com/Oo-site），这是一种用于可视化生殖系分化不同阶段的批量RNA-seq，scRNA-seq和翻译效率数据的工具，这使得非生物信息学家可以访问这些数据。使用这个工具，我们概括了以前报道的发育调控基因的表达模式，并发现减数分裂基因，如那些调节联会复合体，在翻译水平进行调节。总结：我们创建了一个仪表板来可视化果蝇生殖器中的基因表达，并发现减数分裂进入在翻译水平上受到调节。
Determining how stem cell differentiation is controlled has important implications for understanding the etiology of degenerative disease and designing regenerative therapies. In vivo analyses of stem cell model systems have revealed regulatory paradigms for stem cell self-renewal and differentiation. The germarium of the female Drosophila gonad, which houses both germline and somatic stem cells, is one such model system. Bulk mRNA sequencing (RNA-seq), single-cell RNA-seq (scRNA-seq), and bulk translation efficiency (polysome-seq) of mRNAs are available for stem cells and their differentiating progeny within the Drosophila germarium. However, visualizing those data is hampered by the lack of a tool to spatially map gene expression and translational data in the germarium. Here, we have developed Oo-site (https://www.ranganlab.com/Oo-site), a tool for visualizing bulk RNA-seq, scRNA-seq, and translational efficiency data during different stages of germline differentiation, which makes these data accessible to non-bioinformaticians. Using this tool, we recapitulated previously reported expression patterns of developmentally regulated genes and discovered that meiotic genes, such as those that regulate the synaptonemal complex, are regulated at the level of translation. Summary: We created a dashboard to visualize gene expression in the Drosophila germarium and discovered that meiotic entry is regulated at the level of translation.
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影响因子：
11.8
作者：
Carreira-Rosario A;Bhargava V;Hillebrand J;Kollipara RK;Ramaswami M;Buszczak M
通讯作者：
Buszczak M
影响因子：
8.8
作者：
Flora, Pooja;Wong-Deyrup, Siu Wah;Rangan, Prashanth
通讯作者：
Rangan, Prashanth
影响因子：
23.9
作者：
Lehmann, Ruth
通讯作者：
Lehmann, Ruth
影响因子：
1.6
作者：
CARPENTER, ATC
通讯作者：
CARPENTER, ATC
影响因子：
11.8
作者：
Decotto, E;Spradling, AC
通讯作者：
Spradling, AC