Molecular epidemiology of Japanese encephalitis virus in mosquitoes during an outbreak in China, 2013.

Molecular epidemiology of Japanese encephalitis virus in mosquitoes during an outbreak in China, 2013.
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2013年,中国爆发期间,蚊子中日本脑炎病毒的分子流行病学。

DOI:
10.1038/srep04908
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发表时间:
2014-05-09
期刊:
影响因子:
4.6
通讯作者:
Xu A
Xu A
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Tao Z;Liu G;Wang M;Wang H;Lin X;Song L;Wang S;Wang H;Liu X;Cui N;Song Y;Xu A

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日本脑炎病毒(JEV)可引起严重的脑炎,库蚊是主要媒介。 2013年,中国山东省爆发乙脑疫情,确诊病例407例,其中11人死亡。对疫情期间蚊子中的乙型脑炎病毒进行了调查。 3个地点共采集蚊子14,719只。对于 12,695 只三带喙库蚊,RT-PCR 检测结果显示 88/201 份混合样本中存在前膜或包膜蛋白编码基因呈阳性。 3 个地点每 1,000 个媒介中 JEV 阳性个体的最大似然估计值分别为 12.0、7.2 和 6.0,总体估计值为 9.1。对这些前膜 (n = 72) 和包膜 (n = 26) 序列与参考毒株的系统发育分析表明,它们属于基因型 I。这项研究描述了 JEV 的分子流行病学,并表明蚊子的高感染率是爆发的一个重要因素。
Japanese encephalitis virus (JEV) can cause serious encephalitis and Culex mosquitoes are the primary vector. In 2013, a JE outbreak occurred in Shandong Province, China with 407 confirmed cases, including 11 deaths. An investigation on JEV in mosquitoes during the outbreak was conducted. A total of 14,719 mosquitoes were collected at 3 sites. For the 12,695 Culex tritaeniorhynchus mosquitoes, 88/201 pooled samples were positive by RT-PCR for the presence of the pre-membrane or envelope protein coding genes. The maximum likelihood estimates of JEV positive individuals per 1,000 vectors were 12.0, 7.2, and 6.0 in the 3 sites respectively with an overall estimate of 9.1. Phylogenetic analysis on these pre-membrane (n = 72) and envelope (n = 26) sequences with those of reference strains revealed they belonged to genotype I. This study describes the molecular epidemiology of JEV and suggests the high infection rate in mosquitoes is an important factor for the outbreak.
中国西部云南省的蚊子:季节性丰富,多样性和arbovirus协会。
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