Cloning and expression analysis of a hsp70 gene from Saussurea medusa
Cloning and expression analysis of a hsp70 gene from Saussurea medusa
复制标题
水母雪莲hsp70基因的克隆及表达分析
DOI：
10.1080/10425170600609256
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发表时间：
2006-01
期刊：
影响因子：
--
通讯作者：
Zhao, Dexiu
中科院分区：
文献类型：
--
作者：
Fu, Chunxiang;Jin, Zhiping;Cheng, Liqin;Zhao, Dexiu
70 kD热休克基因家族成员在广泛的生物体中高度保守。为了进一步了解极端环境植物水母雪莲（SaussureamedusaMaximHsp 70）的进化和可能的功能，我们从水母雪莲（SaussureamedusaMaximHsp 70）的cDNA文库中克隆了一个编码该蛋白质家族胞浆成员Smhsp 70的cDNA克隆。水母细胞培养物。结果表明，Smhsp 70的cDNA全长为2224 bp，开放阅读框为1941 bp，编码647个氨基酸残基，预测分子量为70，794 Da，C端氨基酸基序为“PKIEEVD”，表明Smhsp 70与高等植物hsp 70家族的胞质成员有亲缘关系。通过分子模拟分析了Smhsp 70的二级和三维结构。Smhsp 70基因组结构包括一个内含子，长度为1134 bp。Smhsp 70蛋白与烟草和番茄的hsp 70蛋白的同源性分别为93.7%和93.2%，与人的hsp 70蛋白的同源性为73.2%，与大肠杆菌的DnaK蛋白的同源性为43.7%。大肠杆菌中表达。半定量逆转录-聚合酶链反应（RT-PCR）分析表明，细胞溶质Smhsp 70蛋白的组成型表达和显着增加后，相对较短的时间内的热休克（37°C），以及低温（4°C）处理。
Members of the 70 kD heat-shock gene family are highly conserved across a wide range of organisms. In an effort to learn more about the evolution and possible functions of extreme environment plant Saussurea medusa Maxim hsp70, we isolated a cDNA clone encoding a putative cytosolic member (Smhsp70) of this family of proteins from a cDNA library of S. medusa cell cultures. The cDNA clone was 2224 bp in length and contained a 1941 bp open reading frame (ORF) encoding a polypeptide of 647 amino acid residues with a predicted molecular mass of 70,794 Da. The predicted protein was found to contain a C-terminal amino acid motif of “PKIEEVD” indicating that Smhsp70 was related to cytosolic members of the hsp70 family in higher plant. The secondary and three-dimensional structures of Smhsp70 were analyzed by molecular modeling. The genomic structure of Smhsp70 included one intron of 1134 bp in length. The deduced Smhsp70 protein has 93.7 and 93.2% similarity with the hsp70 of tobacco and tomato, 73.2% with the hsp70 of human, and 43.7% with DnaK of Escherichia. coli, respectively. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses indicated that the cytosolic Smhsp70 protein was constitutively expressed and markedly increased after relatively short periods of heat shock (37°C) as well as by low temperature (4°C) treatments.
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影响因子：
5.3
作者：
M. Stevenson;S. Calderwood
通讯作者：
M. Stevenson;S. Calderwood
影响因子：
4.8
作者：
Lee, JS;Lee, JJ;Seo, JS
通讯作者：
Seo, JS
DOI：
10.1007/bf00548934
发表时间：
1990-10
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
D. Young
通讯作者：
D. Young
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
DOI：
10.1080/1042517031000154952
发表时间：
2003-10
期刊：
DNA Sequence
影响因子：
--
作者：
S. Sachdev;M. Raychowdhury;S. Sarkar
通讯作者：
S. Sachdev;M. Raychowdhury;S. Sarkar