Single-cell atlas of the first intra-mammalian developmental stage of the human parasite Schistosoma mansoni.
Single-cell atlas of the first intra-mammalian developmental stage of the human parasite Schistosoma mansoni.
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DOI：
10.1038/s41467-020-20092-5
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发表时间：
2020-12-18
影响因子：
16.6
通讯作者：
Berriman M
中科院分区：
文献类型：
--
作者：
Diaz Soria CL;Lee J;Chong T;Coghlan A;Tracey A;Young MD;Andrews T;Hall C;Ng BL;Rawlinson K;Doyle SR;Leonard S;Lu Z;Bennett HM;Rinaldi G;Newmark PA;Berriman M
Over 250 million people suffer from schistosomiasis, a tropical disease caused by parasitic flatworms known as schistosomes. Humans become infected by free-swimming, water-borne larvae, which penetrate the skin. The earliest intra-mammalian stage, called the schistosomulum, undergoes a series of developmental transitions. These changes are critical for the parasite to adapt to its new environment as it navigates through host tissues to reach its niche, where it will grow to reproductive maturity. Unravelling the mechanisms that drive intra-mammalian development requires knowledge of the spatial organisation and transcriptional dynamics of different cell types that comprise the schistomulum body. To fill these important knowledge gaps, we perform single-cell RNA sequencing on two-day old schistosomula of Schistosoma mansoni. We identify likely gene expression profiles for muscle, nervous system, tegument, oesophageal gland, parenchymal/primordial gut cells, and stem cells. In addition, we validate cell markers for all these clusters by in situ hybridisation in schistosomula and adult parasites. Taken together, this study provides a comprehensive cell-type atlas for the early intra-mammalian stage of this devastating metazoan parasite. Schistosomes undergo several develepmental stages during infection of humans. Here, the authors perform single-cell RNA sequencing on the earliest intra-mammalian stage of Schistosoma mansoni and generate a comprehensive cell-type atlas for this human parasite.
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影响因子：
7.2
作者：
Adell, Teresa;Cebria, Francesc;Salo, Emili
通讯作者：
Salo, Emili
影响因子：
64.5
作者：
ARBER, S;HALDER, G;CARONI, P
通讯作者：
CARONI, P
影响因子：
7
作者：
DeMarco, Ricardo;Mathieson, William;Wilson, R. Alan
通讯作者：
Wilson, R. Alan
影响因子：
56.9
作者：
Cao, Junyue;Packer, Jonathan S.;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
6.7
作者：
Collins JJ 3rd;Newmark PA
通讯作者：
Newmark PA