Characterization of small‐field bistratified amacrine cells in macaque retina labeled by antibodies against synaptotagmin‐2
Characterization of small‐field bistratified amacrine cells in macaque retina labeled by antibodies against synaptotagmin‐2
复制标题
突触结合蛋白 2 抗体标记的猕猴视网膜小视场双层无长突细胞的表征
DOI：
10.1002/cne.23201
复制
发表时间：
2013
影响因子：
2.5
通讯作者：
Haverkamp S
中科院分区：
文献类型：
--
作者：
Neumann S;Haverkamp S
用针对蛋白质突触结合蛋白-2（Syt 2）的单克隆抗体对猕猴视网膜进行免疫染色。Syt 2定位于小视野无长突细胞群中，其细胞体在内核层内形成规则的镶嵌，表明它们代表单一的无长突细胞类型。标记的无长突细胞具有双层外观，在OFF层中具有致密的树突丛，并且只有少数小叶突起延伸到内网状层的ON层中，与猫和人视网膜中描述的A8无长突细胞相似。Syt 2标记的细胞对甘氨酸具有免疫反应性，但对γ-氨基丁酸（GABA）缺乏免疫反应性，这表明它们使用甘氨酸作为神经递质。这些细胞的密度从周边视网膜的200个/mm ~ 2增加到中心视网膜的1,400个/mm ~ 2。他们的双极细胞输入进行了研究，使用各种双极细胞标记物结合CtBP 2，标记突触前带的免疫标记实验。我们的数据表明，Syt 2标记的无长突细胞接受来自OFF和ON锥双极细胞以及杆双极细胞的输入。OFF输入由扩散双极单元DB 1（44%）和OFF小型双极单元（38%）主导。在这里，我们描述了一群与A8无长突细胞非常相似的双层小视野无长突细胞及其锥体主导的双极细胞输入。神经学比较杂志521：709-724，2013.© 2012 Wiley Periodicals，Inc.
Macaque retinae were immunostained with monoclonal antibodies directed against the protein synaptotagmin‐2 (Syt2). Syt2 was localized in a population of small‐field amacrine cells, whose cell bodies formed a regular mosaic within the inner nuclear layer, indicating they represent a single amacrine cell type. The labeled amacrine cells had a bistratified appearance with a dense dendritic plexus in the OFF‐layer and only a few lobular processes extending into the ON‐layer of the inner plexiform layer, similar to A8 amacrine cells described in cat and human retina. Syt2‐labeled cells were immunoreactive for glycine but lacked immunoreactivity for γ‐aminobutyric acid (GABA), suggesting they use glycine as their neurotransmitter. The density of these cells increases from ∼200/mm2in peripheral retina to ∼1,400/mm2in central retina. Their bipolar cell input was studied by immunolabeling experiments using various bipolar cell markers combined with CtBP2, a marker of presynaptic ribbons. Our data show that Syt2‐labeled amacrine cells receive input from both OFF and ON cone bipolar cells, as well as from rod bipolar cells. The OFF input is dominated by the diffuse bipolar cell DB1 (44%) and the OFF midget bipolar cell (38%). Here we describe a population of bistratified small‐field amacrine cells closely resembling A8 amacrine cells and their cone‐dominated bipolar cell input. J. Comp. Neurol. 521:709–724, 2013. © 2012 Wiley Periodicals, Inc.
登录
查看更多内容
猕猴视网膜中小型双极细胞的免疫细胞化学特征和空间分布
影响因子：
1.8
作者：
H. Wa¨ssle;Ulrike Gru¨nert;Paul R. Martin;Brian B. Boycotts
通讯作者：
Brian B. Boycotts
影响因子：
2.5
作者：
Hsueh, Hain-Ann;Molnar, Alyosha;Werblin, Frank S.
通讯作者：
Werblin, Frank S.
影响因子：
16.2
作者：
Schmitz, F;Königstorfer, A;Südhof, TC
通讯作者：
Südhof, TC
DOI：
10.1098/rspb.1978.0026
发表时间：
1978-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
WASSLE, H;RIEMANN, HJ
通讯作者：
RIEMANN, HJ
所有无长突细胞限制中央猕猴视网膜的暗视敏锐度：钙视网膜蛋白标记的共聚焦分析
DOI：
--
发表时间：
1999
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
S. Mills;S. Massey
通讯作者：
S. Massey