Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from Schisandra chinensis Based on the Spectrum-Effect Relationship.
Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from Schisandra chinensis Based on the Spectrum-Effect Relationship.
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基于光谱效应的关系，来自Schisandra Chinensis的二苯并杂核木质激素的肝保护作用。
DOI：
10.3390/molecules26216554
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发表时间：
2021-10-29
期刊：
影响因子：
--
通讯作者：
Mu SZ
中科院分区：
文献类型：
--
作者：
Deng LL;Xie XD;Li J;Wang DP;Hao XJ;Chen G;Mu SZ
为科学地阐明五味子的保肝成分，采用最佳提取工艺制备了11批五味子总二苯并环七烯木脂素（TDL）样品。采用高效液相色谱（HPLC）分析技术获得了其特征图谱，并采用MTT法评价了11批TDL的保肝作用。基于化学和生物活性结果，利用Minitab 16.0数据分析软件建立了TDL特征HPLC指纹图谱与其保肝作用的谱效关系。在光谱效应关系的基础上，采用化学天然产物化学分离纯化技术从TDL中分离得到13个化合物（1 ~ 13），并结合光谱数据和文献对其结构进行了鉴定。基于这些化合物，鉴定出上述HPLC指纹图谱中33个色谱峰中的13个共有峰。我们的研究结果表明，一些成分，包括五味子素B(2)，五味子素A(3)和五味子素B(7)在促进肝保护活性方面具有重要作用。初步验证了五倍子TDL的谱效关系，结果证实了这三种关键成分以最佳配比组合的活性优于任何单个化合物，这可能证实了谱效关系和中药增效作用的可靠性。
To scientifically clarify the hepatoprotective constituents of Fructus Schizandrae chinensis, eleven batches samples of total dibenzocyclooctadiene lignans (TDL) from Schisandra chinensis were prepared by using the optimum extraction technique. Characteristic high-performance liquid chromatography (HPLC) chromatograms were obtained through HPLC analysis technology, and the hepatoprotective effects of the eleven batches of TDL were evaluated by MTT assay. Based on the chemical and biological activity results, the spectrum-effect relationship between the characteristic HPLC fingerprints and the hepatoprotective effect of TDL was established using Minitab 16.0 data analysis software. On the basis of the spectrum-effect relationship, thirteen compounds (1–13) were obtained from the TDL by chemical natural product chemical separation and purification technology, and their structures were identified on the basis of the spectral data and the literature. Based on these compounds, thirteen common peaks among the thirty-three chromatographic peaks in the above HPLC fingerprints were identified. Our findings showed that some components, including, schisandrin B (2), schisandrin A (3), and schisandrol B (7) had significant roles in promoting hepatoprotective activity. Preliminary verification of the spectrum-effect relationship of TDL from S. chinensis was carried out, and the results confirmed that the activity of a composite of these three key components in optimal ratios was better than that of any individual compound, which potentially confirmed the reliability of the spectrum-effect relationship and the synergistic effects of traditional Chinese medicine.
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DOI：
10.1155/2017/1352948
发表时间：
2017
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Mohammed Abubakar B;Mohd Salleh F;Shamsir Omar MS;Wagiran A
通讯作者：
Wagiran A
影响因子：
4.6
作者：
Li, Yehong;Zhang, Yiming;Xiong, Yin
通讯作者：
Xiong, Yin
DOI：
10.1007/s11101-016-9470-4
发表时间：
2017
期刊：
Phytochemistry reviews : proceedings of the Phytochemical Society of Europe
影响因子：
--
作者：
Szopa A;Ekiert R;Ekiert H
通讯作者：
Ekiert H
基于网络药理学的五味子保肝作用研究
DOI：
10.3390/molecules22101617
发表时间：
2017-09-28
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Hong M;Zhang Y;Li S;Tan HY;Wang N;Mu S;Hao X;Feng Y
通讯作者：
Feng Y
影响因子：
6.1
作者：
Li, Ling;Pan, Qiangrong;Hu, Xun
通讯作者：
Hu, Xun