The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty.
The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty.
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角膜移植术中供体角膜疱疹病毒DNA阳性的发生率及影响
DOI：
10.1007/s00417-020-04984-2
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Hong J
中科院分区：
文献类型：
--
作者：
Qu JH;Peng RM;Xiao GG;Qu HQ;Yu T;Zhang S;Hong J
目的检测供者角膜中单纯疱疹病毒1型（HSV-1）、单纯疱疹病毒2型（HSV-2）、水痘带状疱疹病毒（VZV）、巨细胞病毒（CMV）和EB病毒（EBV）的DNA，并对病毒阳性的受者进行临床观察。2017年4月至2019年7月通过实时PCR检测EBV。结果23例（2.44%）角膜移植患者疱疹病毒DNA检测呈阳性。HSV-1、CMV、VZV和EBV的阳性率分别为0.74%、0.85%、0.64%和0.21%，respectives.ConclusionWe建议，角膜移植前应检测角膜疱疹病毒DNA。最后，HSV-1可以从移植物传播到受体，但根据我们的观察，CMV不能传播。发现HSV-1阳性的供体角膜必须丢弃，不用于角膜移植术。
PurposeWe detected the DNA of herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in donor corneas and assessed the clinical outcomes of recipients who received virus-positive grafts.MethodAll donor corneas were analyzed for the presence of HSV-1, HSV-2, VZV, CMV, and EBV by real-time PCR from April 2017 to July 2019. The medical records of the transplant patients who received virus-positive grafts were reviewed.ResultTwenty-three (2.44%) donor cornea buttons tested positive for herpesviridae DNA. The positivity rates of HSV-1, CMV, VZV, and EBV were 0.74%, 0.85%, 0.64%, and 0.21%, respectively.ConclusionWe suggest that the corneas from donors who had cancer, donors who were inpatients, and donors who had immunodeficiency or who were on immunosuppressive therapy should be tested for herpesviridae DNA before transplantation. Finally, HSV-1 can be transmitted from graft to recipient, but that CMV cannot be transmitted according to our observations. The donor corneas found to be HSV-1-positive have to be discarded and not used for keratoplasty.
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