A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans.
A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans.
复制标题
DOI：
10.1039/c2lc00001f
复制
发表时间：
2012-06-21
期刊：
影响因子：
6.1
通讯作者：
Weeks JC
中科院分区：
文献类型：
--
作者：
Lockery SR;Hulme SE;Roberts WM;Robinson KJ;Laromaine A;Lindsay TH;Whitesides GM;Weeks JC
本文描述了一种微流控装置的制造和使用，该装置用于使用线虫C.优雅的。该设备由一系列微通道组成，电极连接到微通道上，形成能够检测咽部电活动的记录模块，咽部是一个与进食有关的心脏样神经肌肉器官。该阵列耦合到树状排列的分配通道，该分配通道自动地将一个线虫递送到每个记录模块。然后使用相同的通道向记录模块灌注测试溶液，同时以足够的灵敏度记录来自每个蠕虫的咽电图（EPG），以检测每个咽部收缩。该设备准确地报告了已知驱虫剂（抗线虫药物）的急性作用，并正确区分了一种特定的耐药突变株C。elegans野生型这里描述的方法是很容易适应寄生虫物种的识别新的驱虫药。它也适用于人类代谢和退行性疾病的毒理学和药物发现计划，其中C。elegans被用作模型。
This paper describes the fabrication and use of a microfluidic device for performing whole-animal chemical screens using non-invasive electrophysiological readouts of neuromuscular function in the nematode worm, C. elegans. The device consists of an array of microchannels to which electrodes are attached to form recording modules capable of detecting the electrical activity of the pharynx, a heart-like neuromuscular organ involved in feeding. The array is coupled to a tree-like arrangement of distribution channels that automatically delivers one nematode to each recording module. The same channels are then used to perfuse the recording modules with test solutions while recording the electropharyngeogram (EPG) from each worm with sufficient sensitivity to detect each pharyngeal contraction. The device accurately reported the acute effects of known anthelmintics (anti-nematode drugs) and also correctly distinguished a specific drug-resistant mutant strain of C. elegans from wild type. The approach described here is readily adaptable to parasitic species for the identification of novel anthelmintics. It is also applicable in toxicology and drug discovery programs for human metabolic and degenerative diseases for which C. elegans is used as a model.
登录
查看更多内容
影响因子：
6.1
作者：
Hulme, S. Elizabeth;Shevkoplyas, Sergey S.;Whitesides, George M.
通讯作者：
Whitesides, George M.
影响因子：
--
作者：
Buckingham, Steven D.;Sattelle, David B.
通讯作者：
Sattelle, David B.
影响因子：
6.1
作者：
Chung K;Zhan M;Srinivasan J;Sternberg PW;Gong E;Schroeder FC;Lu H
通讯作者：
Lu H
影响因子：
3.6
作者：
Pemberton, DJ;Franks, CJ;Holden-Dye, L
通讯作者：
Holden-Dye, L
影响因子：
3.3
作者：
Hobson, RJ;Hapiak, VM;Komuniecki, RW
通讯作者：
Komuniecki, RW