Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes.
Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes.
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DOI：
10.1186/1471-2164-12-56
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发表时间：
2011-01-24
期刊：
影响因子：
4.4
通讯作者：
Williamson MS
中科院分区：
文献类型：
--
作者：
Karatolos N;Pauchet Y;Wilkinson P;Chauhan R;Denholm I;Gorman K;Nelson DR;Bass C;ffrench-Constant RH;Williamson MS
温室白粉虱Trialeurodes vaporariorum是温带地区重要的经济作物害虫，对大多数类别的杀虫剂产生了抗性。然而，耐药性的分子机制尚未得到表征，迄今为止，由于缺乏该物种的核苷酸序列数据，进展受到阻碍。在这里，我们在Roche 454-FLX平台上使用焦磷酸测序来产生大量的注释EST数据集。这个“单一基因集”将形成一个关键的参考点，通过数字转录组学定量过度表达的信息。焦磷酸测序产生了大约100万个测序读段，组装成54，748个重叠群，平均长度为965 bp，代表了T. vaporariorum（在本文发表时，GenBank中只有43个条目）。非冗余数据库的BLAST搜索返回了20，333个显著匹配，并且手动管理和注释了可能编码涉及杀虫剂抗性的基因产物的那些基因家族。这些酶包括可能参与异生物质解毒的酶和编码主要化学类别杀虫剂靶标的酶。共鉴定出57个P450、17个GST和27个CCE，沿着编码6种不同杀虫剂类别的靶蛋白的30个重叠群。在这里，我们开发了新的转录组资源T。温室其中包括一个实质性的和注释的EST数据集，将服务于社区研究这一重要的作物害虫，并将进一步阐明杀虫剂抗性的分子机制。
The whitefly Trialeurodes vaporariorum is an economically important crop pest in temperate regions that has developed resistance to most classes of insecticides. However, the molecular mechanisms underlying resistance have not been characterised and, to date, progress has been hampered by a lack of nucleotide sequence data for this species. Here, we use pyrosequencing on the Roche 454-FLX platform to produce a substantial and annotated EST dataset. This 'unigene set' will form a critical reference point for quantitation of over-expressed messages via digital transcriptomics. Pyrosequencing produced around a million sequencing reads that assembled into 54,748 contigs, with an average length of 965 bp, representing a dramatic expansion of existing cDNA sequences available for T. vaporariorum (only 43 entries in GenBank at the time of this publication). BLAST searching of non-redundant databases returned 20,333 significant matches and those gene families potentially encoding gene products involved in insecticide resistance were manually curated and annotated. These include, enzymes potentially involved in the detoxification of xenobiotics and those encoding the targets of the major chemical classes of insecticides. A total of 57 P450s, 17 GSTs and 27 CCEs were identified along with 30 contigs encoding the target proteins of six different insecticide classes. Here, we have developed new transcriptomic resources for T. vaporariorum. These include a substantial and annotated EST dataset that will serve the community studying this important crop pest and will elucidate further the molecular mechanisms underlying insecticide resistance.
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影响因子：
4.4
作者：
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DOI：
10.1073/pnas.0502901102
发表时间：
2005-06-14
影响因子：
11.1
作者：
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通讯作者：
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