Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries.
Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries.
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DOI：
10.1186/1471-2164-7-79
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发表时间：
2006-04-11
期刊：
影响因子：
4.4
通讯作者：
Brown JK
中科院分区：
文献类型：
--
作者：
Leshkowitz D;Gazit S;Reuveni E;Ghanim M;Czosnek H;McKenzie C;Shatters RL Jr;Brown JK
在过去的三十年里，人们对烟粉虱的兴趣急剧增加，这是由于其作为分类学上的神秘物种的性质，由于其专门在韧皮部取食而对大量草本植物造成的损害，以及其作为植物病毒载体的能力。是由B传播的最重要的植物病毒之一。烟粉虱是双生病毒属（双生病毒科）的那些。令人惊讶的是，人们对这种粉虱的基因组知之甚少。雄性B的单倍体基因组大小。通过流式细胞术分析，估计烟粉虱的大小约为10亿bp，约为果蝇Drosophilamelanogaster的5倍。参与粉虱发育、宿主范围可塑性以及菜豆病毒载体特异性和能力的基因尚不清楚。为了解决这种普遍缺乏的基因组序列信息，我们已经构建了三个cDNA文库从non-viruliferous粉虱（鸡蛋，未成熟的龄期，和成人）和两个成年昆虫喂养的番茄植物感染两种双生病毒：番茄黄化曲叶病毒（TYLCV）和番茄斑驳病毒（ToMoV）。总共确定了18，976个克隆的序列。在质量控制和去除5，542个线粒体来源的克隆后，剩下9，110个序列，其中包括3，843个单例和1，017个重叠群。与公共数据库的比较表明，该文库包含参与细胞和发育过程的基因。此外，大约1,000个碱基与B的基因组对齐。烟粉虱内共生细菌黑粉虱Portiera aleyrodidarum，主要来源于卵和龄期文库。除了线粒体序列外，最长和最丰富的序列编码卵黄蛋白原，其来源于粉虱成虫文库，表明这种昆虫中的大部分基因表达是针对卵的产生。这是第一个涉及来自亚热带/热带的半翅目（同翅目）昆虫的功能基因组学项目。B。烟粉虱基因组序列数据库为烟粉虱发育、行为和B相关基因的鉴定提供了重要的工具。烟草介导的菜豆病毒传播。
The past three decades have witnessed a dramatic increase in interest in the whitefly Bemisia tabaci, owing to its nature as a taxonomically cryptic species, the damage it causes to a large number of herbaceous plants because of its specialized feeding in the phloem, and to its ability to serve as a vector of plant viruses. Among the most important plant viruses to be transmitted by B. tabaci are those in the genus Begomovirus (family, Geminiviridae). Surprisingly, little is known about the genome of this whitefly. The haploid genome size for male B. tabaci has been estimated to be approximately one billion bp by flow cytometry analysis, about five times the size of the fruitfly Drosophila melanogaster. The genes involved in whitefly development, in host range plasticity, and in begomovirus vector specificity and competency, are unknown. To address this general shortage of genomic sequence information, we have constructed three cDNA libraries from non-viruliferous whiteflies (eggs, immature instars, and adults) and two from adult insects that fed on tomato plants infected by two geminiviruses: Tomato yellow leaf curl virus (TYLCV) and Tomato mottle virus (ToMoV). In total, the sequence of 18,976 clones was determined. After quality control, and removal of 5,542 clones of mitochondrial origin 9,110 sequences remained which included 3,843 singletons and 1,017 contigs. Comparisons with public databases indicated that the libraries contained genes involved in cellular and developmental processes. In addition, approximately 1,000 bases aligned with the genome of the B. tabaci endosymbiotic bacterium Candidatus Portiera aleyrodidarum, originating primarily from the egg and instar libraries. Apart from the mitochondrial sequences, the longest and most abundant sequence encodes vitellogenin, which originated from whitefly adult libraries, indicating that much of the gene expression in this insect is directed toward the production of eggs. This is the first functional genomics project involving a hemipteran (Homopteran) insect from the subtropics/tropics. The B. tabaci sequence database now provides an important tool to initiate identification of whitefly genes involved in development, behaviour, and B. tabaci-mediated begomovirus transmission.
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影响因子：
1.9
作者：
Brown, J. K.;Lambert, G. M.;Galbraith, D. W.
通讯作者：
Galbraith, D. W.
影响因子：
5.8
作者：
Al-Shahrour, F;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
4.9
作者：
Frohlich, DR;Torres-Jerez, I;Brown, JK
通讯作者：
Brown, JK
影响因子：
3.2
作者：
Ghanim, M;Morin, S;Czosnek, H
通讯作者：
Czosnek, H
影响因子：
14.9
作者：
Drysdale RA;Crosby MA;FlyBase Consortium
通讯作者：
FlyBase Consortium