Role of maternal elimination diets and human milk IgA in the development of cow's milk allergy in the infants.
Role of maternal elimination diets and human milk IgA in the development of cow's milk allergy in the infants.
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DOI：
10.1111/cea.12228
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Berin C
中科院分区：
文献类型：
--
作者：
Järvinen KM;Westfall JE;Seppo MS;James AK;Tsuang AJ;Feustel PJ;Sampson HA;Berin C
母亲避免饮食在预防食物过敏中的作用目前还在争论中。关于这种饮食对母乳（HM）成分或婴儿体液反应诱导的影响知之甚少。评估母乳喂养期间母体牛奶（CM）避免与HM中特定IgA水平和婴儿牛奶过敏（CMA）发展的关系。我们使用了来自145对预期出生队列的HM和婴儿血清样本。采用ELISA法检测母体血清和HM样品中酪蛋白和β -乳球蛋白（BLG）特异性IgA和IgG的含量；21名母亲由于兄弟姐妹有食物过敏史而预防性地开始严格的母体CM避免饮食，16名母亲因婴儿在出生后3个月内出现特应性湿疹或反流/呕吐而开始。检测婴儿血清酪蛋白和blg特异性IgG、IgA和IgE；口服食物挑战证实了CMA。利用Caco-2肠上皮细胞系进行胞吞试验，评估HM对BLG摄取的影响。避免CM的母亲在HM中的酪蛋白和blg特异性IgA低于没有CM限制的母亲（p=0.019和p=0.047）。他们的婴儿血清中酪蛋白和blg特异性IgG1 （p=0.025和p<0.001）和blg特异性IgG4水平（p=0.037）较低，他们的酪蛋白和blg特异性IgA水平比没有CM消除饮食的婴儿低（p=0.003和p=0.007）。较低的cm特异性IgG4和IgA水平反过来与婴儿CMA有关。特异IgA水平高但不低的HM会损害BLG的胞吞作用。母亲避免CM与较低的黏膜特异性IgA水平和婴儿CMA的发展有关。HM IgA可能在防止肠道内过量、不受控制的食物抗原摄取，从而预防CMA中发挥作用。
The role of maternal avoidance diets in the prevention of food allergies is currently under debate. Little is known regarding the effects of such diets on human milk (HM) composition or induction of infant humoral responses. To assess the association of maternal cow’s milk (CM) avoidance during breastfeeding with specific IgA levels in HM and development of cow’s milk allergy (CMA) in infants. We utilized HM and infant serum samples from a prospective birth cohort of 145 dyads. Maternal serum and HM samples were assessed for casein and beta-lactoglobulin (BLG)-specific IgA and IgG by ELISA; 21 mothers prophylactically initiated a strict maternal CM avoidance diet due to a sibling’s history of food allergy and 16 due to atopic eczema or regurgitation/vomiting seen in their infants within the first 3 months of life. Infants’ sera were assessed for casein and BLG-specific IgG, IgA and IgE; CMA was confirmed by an oral food challenge. The impact of HM on BLG uptake was assessed in transcytosis assays utilizing Caco-2 intestinal epithelial cell line. Mothers avoiding CM had lower casein- and BLG-specific IgA in HM than mothers with no CM restriction (p=0.019 and p=0.047). Their infants had lower serum casein- and BLG-specific IgG1 (p=0.025 and p<0.001) and BLG-specific IgG4 levels (p=0.037) and their casein- and BLG-specific IgA levels were less often detectable than those with no CM elimination diet (p=0.003 and p=0.007). Lower CM-specific IgG4 and IgA levels in turn were associated with infant CMA. Transcytosis of BLG was impaired by HM with high, but not low levels of specific IgA. Maternal CM avoidance was associated with lower levels of mucosal specific IgA levels and development of CMA in infants. HM IgA may play a role in preventing excessive, uncontrolled food antigen uptake in the gut lumen and thereby in the prevention of CMA.
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影响因子：
2.8
作者：
Kuitunen, Mikael;Kukkonen, Anna Kaarina;Savilahti, Erkki
通讯作者：
Savilahti, Erkki
影响因子：
30.3
作者：
Peterson, Daniel A.;McNulty, Nathan P.;Gordon, Jeffrey I.
通讯作者：
Gordon, Jeffrey I.
影响因子：
4.4
作者：
Mantis, NJ;Cheung, MC;Neutra, MR
通讯作者：
Neutra, MR
DOI：
10.1053/j.spid.2004.07.002
发表时间：
2004-10-01
期刊：
Seminars in pediatric infectious diseases
影响因子：
--
作者：
Morrow, Ardythe L;Rangel, Josefa M
通讯作者：
Rangel, Josefa M
DOI：
10.1053/siny.2002.0124
发表时间：
2002-08-01
期刊：
Seminars in neonatology : SN
影响因子：
--
作者：
Hanson, Lars A;Korotkova, Marina
通讯作者：
Korotkova, Marina