Cell-cell interaction by mouse limb cells during in vitro chondrogenesis: analysis of the brachypod mutation.
Cell-cell interaction by mouse limb cells during in vitro chondrogenesis: analysis of the brachypod mutation.
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体外软骨形成过程中小鼠肢体细胞的细胞间相互作用：短足动物突变分析。
DOI：
10.1016/0012-1606(82)90043-4
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发表时间：
1982
影响因子：
2.7
通讯作者：
Solursh,M
中科院分区：
文献类型：
--
作者：
Owens,EM;Solursh,M
本文包含的观察和实验，共同证明了软骨分化过程中肢芽间充质细胞之间的细胞-细胞相互作用的要求。肢芽细胞分离brachypodismH（bpH）和野生型小鼠胚胎之间的Thieler阶段16-17和中期21进行了比较，他们的能力进行软骨分化的高密度micromass文化。由解离的第12天（第20阶段）bpH肢芽细胞形成的结节先前已报道在大小和数量上异常减少，并且形成延迟。我们证实了这些结果，但发现从早期肢芽（阶段16-17和早期阶段21）制备的bph培养物逐渐更像野生型培养物。在72小时的阶段16- 17 bph培养物实际上含有正常数量和大小的结节，而阶段18 bph培养物在结节形成中介于阶段16-17和阶段21之间。另一方面，我们还发现，即使在由第20阶段培养物制备的bph培养物中，聚集体形成的初始速率也是正常的，其中结节形成不正常。制备主要由早期21 bp肢芽细胞与少量（例如，5%）的16-17期野生型肢芽细胞显示出软骨结节形成的显著增加，超过仅由早期21 bph细胞组成的对照培养物。从大于16-17期的胚胎中获得的野生型细胞的比例更大，需要相同程度的正常化，支持一种特定的细胞类型的假设，其比例随着时间的推移在肢芽中正常下降，需要增加体外软骨形成的bph细胞。此外，以1：20的比例含有23期鸡肢细胞和早期21 bp细胞的培养物每平方毫米培养物含有野生型水平的结节。因此，bpHcells似乎对来自正常肢体间充质细胞的软骨形成诱导信号有反应。为了测试bph肢芽间充质诱导软骨发生的能力，将在培养物中形成相同数量的聚集体和结节的16- 17 bph阶段和野生型肢芽细胞各自与早期21 bph细胞以1：20、1：10和1：3的比例混合。尽管低比例的野生型17期细胞显著增加了这些混合培养物中聚集体和结节的数量，但低比例的bpH 16-17期细胞没有。因此，可以得出结论，bpH突变的主要缺陷可能在于特定间充质细胞亚群提供软骨形成诱导刺激的能力降低。
This paper contains observations and experiments which collectively demonstrate a requirement for cell-cell interactions among limb bud mesenchyme cells during chondrogenic differentiation. Limb bud cells isolated from brachypodismH(bpH) and wild-type mouse embyros between Thieler stage 16–17 and midstage 21 were compared with respect to their abilities to undergo chondrogenic differentiation in high-density micromass cultures. Nodules formed by dissociated Day 12 (stage 20)bpHlimb bud cells have been reported previously to be abnormally reduced in size and number, and delayed in formation. We corroborate these results, but find thatbpHcultures prepared from earlier-stage limb buds (between stages 16–17 and early stage 21) are progressively more like wild-type cultures. Stage 16–17bpHcultures at 72 hr actually contain normal numbers of and size nodules, while stage 18bpHcultures are intermediate between stages 16–17 and stage 21 in nodule formation. On the other hand, we also find that the initial rate of aggregate formation is normal even inbpHcultures prepared from stage 20 cultures in which nodule formation is not normal. Preparation of cultures composed primarily of early stage 21bpHlimb bud cells mixed with small quantities (e.g., 5%) of stage 16–17 wild-type limb bud cells showed significant increases in cartilage nodule formation over control cultures composed only of early stage 21bpHcells. Greater proportions of wild-type cells obtained from embryos older than stages 16–17 were required for the same degree of normalization, supporting the hypothesis that a specific cell type, whose proportion decreases normally in the limb bud over time, is required to increasein vitrochondrogenesis bybpHcells. Additionally, cultures containing stage 23 chick limb cells and early stage 21bpHcells at a ratio of 1:20 contained wild-type levels of nodules per square millimeter of culture. Thus,bpHcells appear to respond to chondrogenic inductive signals from normal limb mesenchyme cells. In order to test for the ability ofbpHlimb bud mesenchyme toinducechondrogenesis, stage 16–17bpHand wild-type limb bud cells, which form identical numbers of aggregates and nodules in culture, were each mixed with early stage 21bpHcells at ratios of 1:20, 1:10, and 1:3. Although low proportions of wild-type stage 17 cells significantly increased the number of aggregates and nodules in these mixed cultures, low proportions ofbpHstage 16–17 cells did not. It is, therefore, concluded that the primary defect of thebpHmutation is likely to reside in the reduced ability of a specific mesenchyme cell subpopulation to provide an inductive stimulus for chondrogenesis.
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影响因子：
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作者：
E. Matsutani;Y. Kuroda
通讯作者：
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DOI：
10.3181/00379727-92-22495
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影响因子：
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DOI：
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发表时间：
1978
期刊：
Journal of embryology and experimental morphology
影响因子：
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作者：
J. Duke;W. A. Elmer
通讯作者：
W. A. Elmer