Combining the high-dose/refuge strategy and self-limiting transgenic insects in resistance management-A test in experimental mesocosms.
Combining the high-dose/refuge strategy and self-limiting transgenic insects in resistance management-A test in experimental mesocosms.
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DOI：
10.1111/eva.12573
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发表时间：
2018-06
影响因子：
4.1
通讯作者：
Raymond B
中科院分区：
文献类型：
--
作者：
Zhou L;Alphey N;Walker AS;Travers LM;Hasan F;Morrison NI;Bonsall MB;Raymond B
高剂量/避难所策略一直是用苏云金芽孢杆菌毒素工程改造的转基因作物抗性管理的主要方法。然而，种植者不断施加压力，要求减少Bt毒素无庇护所的规模，因为这些庇护所通常遭受害虫的更高损害。一种补充方法是释放具有雌性特异性自限性基因的雄性转基因昆虫。这项技术可以减少种群规模，并通过雄性渗入易感基因来减缓抗性的进化。理论预测，它可以用来促进较小的避难所或逆转抗性的进化。在这项研究中，我们使用笼中昆虫种群的实验进化来研究自限性系统和高剂量/避难所策略在减轻小菜蛾抗性进化中的兼容性。自我限制系统的好处在避难所规模较小的情况下更加明显，特别是当避难所不足以防止抗性的演变时。结果表明，转基因雄蚊在笼中能抑制种群规模，延缓抗性发展，其避难所率为10%，初始抗性等位基因频率为4%-15%。半合子转基因昆虫的适合度成本对于将易感等位基因渐渗到目标种群中特别重要。本研究中的自限性基因（小菜蛾OX 4139品系L）的适合度成本是不完全显性的，并且降低了繁殖力和雄性交配竞争力。这里使用的实验进化方法说明了自限性昆虫的大规模释放和高剂量/避难所策略相结合的一些好处和陷阱，但确实表明它们可以互补。
The high‐dose/refuge strategy has been the primary approach for resistance management in transgenic crops engineered with Bacillus thuringiensis toxins. However, there are continuing pressures from growers to reduce the size of Bt toxin‐free refugia, which typically suffer higher damage from pests. One complementary approach is to release male transgenic insects with a female‐specific self‐limiting gene. This technology can reduce population sizes and slow the evolution of resistance by introgressing susceptible genes through males. Theory predicts that it could be used to facilitate smaller refugia or reverse the evolution of resistance. In this study, we used experimental evolution with caged insect populations to investigate the compatibility of the self‐limiting system and the high‐dose/refuge strategy in mitigating the evolution of resistance in diamondback moth, Plutella xylostella. The benefits of the self‐limiting system were clearer at smaller refuge size, particularly when refugia were inadequate to prevent the evolution of resistance. We found that transgenic males in caged mesocosms could suppress population size and delay resistance development with 10% refugia and 4%–15% initial resistance allele frequency. Fitness costs in hemizygous transgenic insects are particularly important for introgressing susceptible alleles into target populations. Fitness costs of the self‐limiting gene in this study (P. xylostella OX4139 line L) were incompletely dominant, and reduced fecundity and male mating competitiveness. The experimental evolution approach used here illustrates some of the benefits and pitfalls of combining mass release of self‐limiting insects and the high‐dose/refuge strategy, but does indicate that they can be complementary.
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影响因子：
3.7
作者：
Gassmann AJ;Petzold-Maxwell JL;Keweshan RS;Dunbar MW
通讯作者：
Dunbar MW
影响因子：
4.1
作者：
Campagne P;Smouse PE;Pasquet R;Silvain JF;Le Ru B;Van den Berg J
通讯作者：
Van den Berg J
影响因子：
3.8
作者：
Carvalho DO;McKemey AR;Garziera L;Lacroix R;Donnelly CA;Alphey L;Malavasi A;Capurro ML
通讯作者：
Capurro ML
DOI：
10.1098/rspb.2008.0846
发表时间：
2008-12-22
影响因子：
4.7
作者：
Gould, Fred;Huang, Yunxin;Lloyd, Alun L.
通讯作者：
Lloyd, Alun L.
影响因子：
5.4
作者：
Harvey-Samuel T;Morrison NI;Walker AS;Marubbi T;Yao J;Collins HL;Gorman K;Davies TG;Alphey N;Warner S;Shelton AM;Alphey L
通讯作者：
Alphey L