Multi-modal molecular diffuse optical tomography system for small animal imaging.
Multi-modal molecular diffuse optical tomography system for small animal imaging.
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DOI：
10.1088/0957-0233/24/10/105405
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发表时间：
2013
影响因子：
2.4
通讯作者：
Dehghani H
中科院分区：
文献类型：
--
作者：
Guggenheim JA;Basevi HR;Frampton J;Styles IB;Dehghani H
提出了一种用于定量三维生物发光和功能漫射成像的多模态光学成像系统，该系统无运动部件，利用反射镜提供多视角层析数据用于图像重建。研究表明，通过使用透射光谱近红外测量和光谱约束层析成像重建，吸收剂的回收浓度可以用作可见光谱内生物发光成像的先验知识。此外，还展示了最近开发的多视图光学表面捕获技术的首次使用，并演示了其在基于模型的图像重建和自由空间光建模中的应用。与二维平面成像相比，基于模型的层析成像图像恢复的好处在许多场景中突出显示，其中内部发光源不可见或在二维图像中混淆。结果表明，发光层析成像方法可以在老鼠大小的实体幻影中产生单个光源的3D重建，在目标位置和深度范围内精确定位到1.5mm以内，表明整个幻影体积的灵敏度和准确成像。此外，重建的总发光源强度在15%以内，与标准生物发光成像相比有了显着的提高。最后，介绍了具有吸收异常的异质幻影的结果，展示了多视图、光谱约束耦合成像系统的使用和优点，该系统可以提供精确的3D发光图像。
A multi-modal optical imaging system for quantitative 3D bioluminescence and functional diffuse imaging is presented, which has no moving parts and uses mirrors to provide multi-view tomographic data for image reconstruction. It is demonstrated that through the use of trans-illuminated spectral near infrared measurements and spectrally constrained tomographic reconstruction, recovered concentrations of absorbing agents can be used as prior knowledge for bioluminescence imaging within the visible spectrum. Additionally, the first use of a recently developed multi-view optical surface capture technique is shown and its application to model-based image reconstruction and free-space light modelling is demonstrated. The benefits of model-based tomographic image recovery as compared to 2D planar imaging are highlighted in a number of scenarios where the internal luminescence source is not visible or is confounding in 2D images. The results presented show that the luminescence tomographic imaging method produces 3D reconstructions of individual light sources within a mouse-sized solid phantom that are accurately localised to within 1.5mm for a range of target locations and depths indicating sensitivity and accurate imaging throughout the phantom volume. Additionally the total reconstructed luminescence source intensity is consistent to within 15% which is a dramatic improvement upon standard bioluminescence imaging. Finally, results from a heterogeneous phantom with an absorbing anomaly are presented demonstrating the use and benefits of a multi-view, spectrally constrained coupled imaging system that provides accurate 3D luminescence images.
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影响因子：
--
作者：
Dehghani, Hamid;Eames, Matthew E.;Yalavarthy, Phaneendra K.;Davis, Scott C.;Srinivasan, Subhadra;Carpenter, Colin M.;Pogue, Brian W.;Paulsen, Keith D.
通讯作者：
Paulsen, Keith D.
影响因子：
3.8
作者：
Basevi, Hector R. A.;Guggenheim, James A.;Styles, Iain B.
通讯作者：
Styles, Iain B.
影响因子：
7.6
作者：
Lv Y;Tian J;Cong W;Wang G
通讯作者：
Wang G
影响因子：
2.8
作者：
Gulsen, Gultekin;Birgul, Ozlem;Nalcioglu, Orhan
通讯作者：
Nalcioglu, Orhan
影响因子：
3.5
作者：
Chaudhari, AJ;Darvas, F;Leahy, RM
通讯作者：
Leahy, RM