Genetic variability of West Nile virus in US blood donors, 2002-2005.
Genetic variability of West Nile virus in US blood donors, 2002-2005.
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DOI：
10.3201/eid1403.070463
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发表时间：
2008-03
影响因子：
11.8
通讯作者：
Rios M
中科院分区：
文献类型：
--
作者：
Grinev A;Daniel S;Stramer S;Rossmann S;Caglioti S;Rios M
随着其地理分布的扩大，这种病毒正在与前体分离株发生分化。西尼罗河病毒 (WNV) 于 1999 年在美国被发现，此后每年夏天都会再次出现，并已成为地方性流行病。 2002 年记录了输血传播，并于 2003 年开始对献血者进行西尼罗病毒筛查。我们研究了从 2002 年至 2005 年期间西尼罗病毒 RNA 检测呈阳性的 30 名受感染的献血者采集的样本中获得的人类分离株中西尼罗河病毒的遗传变异。分析了 8 个分离株的完整基因组序列和另外 22 个分离株的结构基因序列。我们发现来自不同地理区域的分离株存在一定的遗传多样性，并且与 1999-2001 年流行病报告的序列存在遗传差异。结构基因的核苷酸差异从2002年（0.18%）到2005年（0.37%）小幅增加，表明该时期西尼罗河病毒不存在强选择压力且遗传进化有限。然而，随着病毒地理分布的扩大，西尼罗河病毒继续与前体分离株发生分化。
This virus is diverging from precursor isolates as its geographic distribution expands. West Nile virus (WNV) was detected in the United States in 1999, has reoccurred every summer since, and has become endemic. Transfusion transmission was documented in 2002, and screening of blood donations for WNV began in 2003. We investigated genetic variation of WNV in human isolates obtained from specimens collected from 30 infected blood donors who tested positive for WNV RNA during 2002–2005. Complete genomic sequences of 8 isolates and structural gene sequences from 22 additional isolates were analyzed. We found some genetic diversity in isolates from different geographic regions and genetic divergence from reported sequences from epidemics in 1999–2001. Nucleotide divergence of structural genes showed a small increase from 2002 (0.18%) to 2005 (0.37%), suggesting absence of strong selective pressure and limited genetic evolution of WNV during that period. Nevertheless, WNV has continued to diverge from precursor isolates as geographic distribution of the virus has expanded.
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影响因子：
11.8
作者：
Davis, C Todd;Beasley, David W C;Guzman, Hilda;Raj, Rushker;D'Anton, Mary;Novak, Robert J;Unnasch, Thomas R;Tesh, Robert B;Barrett, Alan D T
通讯作者：
Barrett, Alan D T
影响因子：
3.7
作者：
Beasley, DWC;Davis, CT;Barrett, ADT
通讯作者：
Barrett, ADT
影响因子：
3.5
作者：
Hughes, Austin L.;Piontkivska, Helen;Foppa, Ivo
通讯作者：
Foppa, Ivo
影响因子：
11.8
作者：
Klenk, K;Snow, J;Bunning, M
通讯作者：
Bunning, M
影响因子：
3.8
作者：
Jerzak, G;Bernard, KA;Ebel, GD
通讯作者：
Ebel, GD