Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis.
Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis.
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DOI：
10.3389/fvets.2021.756764
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发表时间：
2021
影响因子：
3.2
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Zhang K;Chu P;Song S;Yang D;Bian Z;Li Y;Gou H;Jiang Z;Cai R;Li C
副猪嗜血杆菌（Haemophilus parasuis，H. parasuis）是目前养猪业中的一种重要病原菌。副猪链球菌可与多种病原微生物共感染猪，并进一步导致疾病的加重。研究其发病机制至关重要。革兰氏阴性菌天然分泌外膜囊泡（OMV），其强毒力因子在影响细菌与宿主相互作用中起重要作用。尽管如此，与细菌OMV相关的发病机制尚未得到很好的阐明。在这项研究中，我们研究了分泌OMV从临床H。副猪链球菌分离株（H45）。此外，我们还进一步分析了OMV的特征、全面的蛋白质组和毒力潜力。我们的数据表明H.在感染过程中，副猪链球菌可将OMV分泌到细胞外环境中。利用液相色谱-串联质谱（MS/MS）鉴定和生物信息分析，我们确定了588个不同的蛋白质与OMV。同时，我们还分析了这些蛋白的亚细胞定位和生物学功能。这些蛋白质主要参与免疫和铁代谢。进一步证实了H. 1和猪肺泡巨噬细胞中的强烈炎症反应。综上所述，我们的研究结果表明，OMV从H。在感染过程中，副猪链球菌参与了该细菌的致病过程。
Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of H. parasuis OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from H. parasuis were involved in the pathogenesis of this bacterium during infection.
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