Differences in phagocytosis susceptibility in Haemophilus parasuis strains.
Differences in phagocytosis susceptibility in Haemophilus parasuis strains.
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DOI：
10.1051/vetres/2009007
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发表时间：
2009-05
影响因子：
4.4
通讯作者：
Aragon V
中科院分区：
文献类型：
--
作者：
Olvera A;Ballester M;Nofrarías M;Sibila M;Aragon V
副猪嗜血杆菌是健康猪上呼吸道的定植菌，但强毒株可引起全身性感染，其特征为化脓性多浆膜炎，通常称为格拉瑟病。在H.目前对副猪嗜血杆菌的毒力机制还不完全清楚。副猪群在很大程度上未知。在感染过程中，H.副猪嗜血杆菌必须在宿主肺防御（包括肺泡巨噬细胞）中存活，以产生疾病。使用来自不同临床背景的菌株，我们能够检测到对吞噬作用敏感性的明显差异。从健康动物的鼻子中分离的菌株被猪肺泡巨噬细胞（PAM）有效地吞噬，而从全身病变中分离的菌株对这种相互作用具有抗性。敏感菌株的吞噬作用通过独立于特定受体的机制进行，该受体涉及肌动蛋白丝和微管。在本研究中测试的所有系统性菌株中，我们在与PAM相互作用后观察到明显的胶囊，表明这种表面结构在抗吞噬作用中的作用。然而，由于我们检测到系统性菌株中微管抑制的不同作用，因此应探索抗吞噬作用的其他机制。
Haemophilus parasuis is a colonizer of the upper respiratory tract of healthy pigs, but virulent strains can cause a systemic infection characterized by fibrinous polyserositis, commonly known as Glässer’s disease. The variability in virulence that is observed among H. parasuis strains is not completely understood, since the virulence mechanisms of H. parasuis are largely unknown. In the course of infection, H. parasuis has to survive the host pulmonary defences, which include alveolar macrophages, to produce disease. Using strains from different clinical backgrounds, we were able to detect clear differences in susceptibility to phagocytosis. Strains isolated from the nose of healthy animals were efficiently phagocytosed by porcine alveolar macrophages (PAM), while strains isolated from systemic lesions were resistant to this interaction. Phagocytosis of susceptible strains proceeded through mechanisms independent of a specific receptor, which involved actin filaments and microtubules. In all the systemic strains tested in this study, we observed a distinct capsule after interaction with PAM, indicating a role of this surface structure in phagocytosis resistance. However, additional mechanisms of resistance to phagocytosis should be explored, since we detected different effects of microtubule inhibition among systemic strains.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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