Membrane adsorbers comprising grafted glycopolymers for targeted lectin binding.
Membrane adsorbers comprising grafted glycopolymers for targeted lectin binding.
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DOI：
10.1002/app.41437
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发表时间：
2015-06-05
影响因子：
3
通讯作者：
Husson, Scott M.
中科院分区：
文献类型：
--
作者：
Chenette, Heather C. S.;Husson, Scott M.
这项工作详细介绍了凝集素净化的亲和膜吸附剂的设计和测试，包括含葡萄糖的糖共聚物。正是糖共聚物的糖残基与凝集素的互补碳水化合物结合结构域之间的选择性相互作用，为从复杂的生物培养基中分离和纯化凝集素提供了基础。在这些研究中使用的设计方法是通过原子转移自由基聚合从大孔再生纤维素膜上接枝糖共聚物“触须”。如早期研究所示，这种设计方法可用于制备高效膜吸附剂。模型凝集素conA （conA）用于评价膜在结合和洗脱纯化中的性能，使用低分子量糖进行洗脱。在平衡和动态条件下，以0.1和1.0 mL/min的流速测量膜结合conA的能力。估计了第一Damkohler数，将吸附速率与通过膜床的对流质输运速率联系起来。它被用来评估吸附动力学或质量传递是否是conA结合的主要限制因素。分析表明，该体系不受结合位点的可及性的限制，而是受conA在糖共聚物上的固有吸附速率的限制。
This work details the design and testing of affinity membrane adsorbers for lectin purifications that incorporate glucose-containing glycopolymers. It is the selective interaction between the sugar residues of the glycopolymer and the complementary carbohydrate-binding domain of the lectin that provides the basis for the isolation and purification of lectins from complex biological media. The design approach used in these studies was to graft glycopolymer ‘tentacles’ from macroporous regenerated cellulose membranes by atom transfer radical polymerization. As shown in earlier studies, this design approach can be used to prepare high-productivity membrane adsorbers. The model lectin, concanavalin A (conA), was used to evaluate membrane performance in bind-and-elute purification, using a low molecular weight sugar for elution. The membrane capacity for binding conA was measured at equilibrium and under dynamic conditions using flow rates of 0.1 and 1.0 mL/min. The first Damkohler number was estimated to relate the adsorption rate to the convective mass transport rate through the membrane bed. It was used to assess whether adsorption kinetics or mass transport contributed the primary limitation to conA binding. Analyses indicate that this system is not limited by the accessibility of the binding sites, but by the inherent rate of adsorption of conA onto the glycopolymer.
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影响因子：
3.7
作者：
LIU, HC;FRIED, JR
通讯作者：
FRIED, JR
影响因子：
3.8
作者：
Bhut, Bharat V.;Weaver, Justin;Husson, Scott M.
通讯作者：
Husson, Scott M.
影响因子：
3.8
作者：
Bhut, Bharat V.;Weaver, Justin;Husson, Scott M.
通讯作者：
Husson, Scott M.
影响因子：
9.5
作者：
Himstedt, Heath H.;Qian, Xianghong;Wickramasinghe, S. Ranil
通讯作者：
Wickramasinghe, S. Ranil
影响因子：
6.2
作者：
Jain, Parul;Vyas, Mukesh Kumar;Geiger, James H.;Baker, Gregory L.;Bruening, Merlin L.
通讯作者：
Bruening, Merlin L.