In vivo measurement of cell-type-specific synaptic connectivity and synaptic transmission in layer 2/3 mouse barrel cortex.
In vivo measurement of cell-type-specific synaptic connectivity and synaptic transmission in layer 2/3 mouse barrel cortex.
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DOI：
10.1016/j.neuron.2014.11.025
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发表时间：
2015-01-07
期刊：
影响因子：
16.2
通讯作者：
Petersen, Carl C. H.
中科院分区：
文献类型：
--
作者：
Pala, Aurelie;Petersen, Carl C. H.
体外膜电位的细胞内记录定义了突触通讯的基本特性。人们对体内突触连接和突触传递的特性知之甚少。在这里，我们将单细胞光遗传学与全细胞记录相结合，研究体内谷氨酸突触传递，从单个识别的兴奋性神经元到 2/3 层小鼠桶状皮层中两种基因定义的抑制性 GABA 能神经元亚型。我们发现表达小清蛋白（PV）GABA能神经元比表达生长抑素（Sst）GABA能神经元接收单一谷氨酸能突触输入的概率更高。 PV 神经元上的单一兴奋性突触后电位也比 Sst 神经元上的输入更快、更可靠。针对 Sst 神经元的兴奋性突触表现出强烈的短期促进作用，而针对 PV 神经元的兴奋性突触则表现出很少的短期动态。我们的结果与体外测量结果基本一致。因此，我们证明了评估体内功能性细胞类型特异性突触连接的技术可行性，从而允许未来研究突触传递的上下文依赖性调节。单细胞光遗传学用于精确刺激体内动作电位 来自遗传定义的突触后 GABA 能神经元的体内全细胞记录 表达小白蛋白的神经元接收强、快速且可靠的兴奋性输入 表达生长抑素的神经元接收更持久、促进兴奋性输入 Pala 和 Petersen 使用单细胞光遗传学和双光子靶向全细胞记录来测量体内突触连接和突触传递从兴奋性神经元到小鼠桶状皮层 2/3 层中表达小清蛋白和表达生长抑素的 GABA 能神经元。
Intracellular recordings of membrane potential in vitro have defined fundamental properties of synaptic communication. Much less is known about the properties of synaptic connectivity and synaptic transmission in vivo. Here, we combined single-cell optogenetics with whole-cell recordings to investigate glutamatergic synaptic transmission in vivo from single identified excitatory neurons onto two genetically defined subtypes of inhibitory GABAergic neurons in layer 2/3 mouse barrel cortex. We found that parvalbumin-expressing (PV) GABAergic neurons received unitary glutamatergic synaptic input with higher probability than somatostatin-expressing (Sst) GABAergic neurons. Unitary excitatory postsynaptic potentials onto PV neurons were also faster and more reliable than inputs onto Sst neurons. Excitatory synapses targeting Sst neurons displayed strong short-term facilitation, while those targeting PV neurons showed little short-term dynamics. Our results largely agree with in vitro measurements. We therefore demonstrate the technical feasibility of assessing functional cell-type-specific synaptic connectivity in vivo, allowing future investigations into context-dependent modulation of synaptic transmission. Single-cell optogenetics for precise stimulation of action potentials in vivo In vivo whole-cell recordings from genetically defined postsynaptic GABAergic neurons Parvalbumin-expressing neurons receive strong, fast, and reliable excitatory input Somatostatin-expressing neurons receive longer-lasting, facilitating excitatory input Pala and Petersen use single-cell optogenetics and two-photon targeted whole-cell recordings to measure synaptic connectivity and synaptic transmission in vivo from excitatory neurons onto parvalbumin-expressing and somatostatin-expressing GABAergic neurons in layer 2/3 of mouse barrel cortex.
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影响因子：
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作者：
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