End-to-Side Neurorrhaphy as Schwann Cells Provider to Acellular Nerve Allograft and Its Suitable Application.
End-to-Side Neurorrhaphy as Schwann Cells Provider to Acellular Nerve Allograft and Its Suitable Application.
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DOI：
10.1371/journal.pone.0167507
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hayashi A
中科院分区：
文献类型：
--
作者：
Yoshizawa H;Senda D;Natori Y;Tanaka R;Mizuno H;Hayashi A
轴突再生依赖于宿主雪旺细胞（SC）的支持，而先前对脱细胞神经同种异体移植物（ANG）的研究表明，轴突可以在有限的距离内再生为ANG。大量研究表明，在ANG中补充外源性因子，如培养的SC、干细胞和生长因子，可促进ANG中的神经再生。然而，存在与它们的使用相关的若干问题。在这项研究中，我们调查了端侧（ETS）神经缝合术，这是一个轴突供应商，是否可以作为一个SC供应商，以支持轴突延长在ANG有用。我们发现，当进行神经外膜窗联合部分神经切除术时，ETS神经缝合术有效地促进SC迁移到ANG中，并且当双侧应用时，有效性会增加。当我们通过ETS神经缝合术将含有迁移的SC的ANG（混合ANG）移植到神经间隙时，混合ANG增加了再生轴突的数量并促进了轴突的快速伸长，特别是当ETS神经缝合术应用于移植物的两个边缘时。这种方法可能代表了ETS神经缝合术的一种新应用，并导致混合型ANG的发展，使ANG在临床环境中更实用。
Axonal regeneration relies on support from proliferating host Schwann cells (SCs), and previous studies on acellular nerve allografts (ANGs) suggest that axons can regenerate into ANGs within a limited distance. Numerous studies have demonstrated that the supplementation of ANGs with exogenous factors, such as cultured SCs, stem cells, and growth factors, promote nerve regeneration in ANGs. However, there are several problems associated with their utilization. In this study, we investigated whether end-to-side (ETS) neurorrhaphy, which is an axonal provider, could be useful as an SC provider to support axonal elongation in ANGs. We found that ETS neurorrhaphy effectively promoted SC migration into ANGs when an epineurium window combined with partial neurectomy was performed, and the effectiveness increased when it was applied bilaterally. When we transplanted ANGs containing migrated SCs via ETS neurorrhaphy (hybrid ANGs) to the nerve gap, hybrid ANGs increased the number of regenerated axons and facilitated rapid axonal elongation, particularly when ETS neurorrhaphy was applied to both edges of the graft. This approach may represent a novel application of ETS neurorrhaphy and lead to the development of hybrid ANGs, making ANGs more practical in a clinical setting.
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DOI：
10.1097/01.jnen.0000171650.94341.46
发表时间：
2005-07-01
影响因子：
3.2
作者：
Chen, YY;McDonald, D;Zochodne, DW
通讯作者：
Zochodne, DW
DOI：
10.1111/j.1365-2990.1986.tb00151.x
发表时间：
1986-07-01
影响因子：
5
作者：
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影响因子：
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影响因子：
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作者：
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影响因子：
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